Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus.

Autor: Vasconcelos SECB; Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil., Melo HM; Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil., Cavalcante TTA; Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil., Júnior FEAC; Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil.; State University of the Tocantina Region of Maranhão, Imperatriz, MA, 65901-480, Brazil., de Carvalho MG; Chemistry Department, Federal Rural University of Rio de Janeiro, Seropédica-RJ, Rio de Janeiro, 23890-000, Brazil., Menezes FGR; Sea Sciences Institute, Federal University of Ceará, Fortaleza, CE, 60165-081, Brazil., de Sousa OV; Sea Sciences Institute, Federal University of Ceará, Fortaleza, CE, 60165-081, Brazil., Costa RA; Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil. renata.albuq@gmail.com.
Jazyk: angličtina
Zdroj: BMC complementary and alternative medicine [BMC Complement Altern Med] 2017 Sep 16; Vol. 17 (1), pp. 462. Date of Electronic Publication: 2017 Sep 16.
DOI: 10.1186/s12906-017-1968-9
Abstrakt: Background: The emergence of multidrug-resistant bacteria is a worldwide concern and in order to find an alternative to this problem, the occurrence of antimicrobial compounds in Plectranthus amboinicus essential oil was investigated. Thus, this study aims to determine susceptibility of Staphylococcus aureus isolated from food to antibiotics, P. amboinicus essential oil (PAEO) and carvacrol.
Methods: Leaves and stem of P. amboinicus were used for extraction of essential oil (PAEO) by hydrodistillation technique and EO chemical analysis was performed by gas chromatography coupled to a mass spectrometer. S. aureus strains (n = 35) isolated from food and S. aureus ATCC 6538 were used to evaluate the antimicrobial and antibiofilm activity of PAEO and carvacrol. All strains (n = 35) were submitted to antimicrobial susceptibility profile by disk diffusion method. Determination of MIC and MBC was performed by microdilution technique and antibiofilm activity was determined by microtiter-plate technique with crystal violet assay and counting viable cells in Colony Forming Units (CFU).
Results: Carvacrol (88.17%) was the major component in the PAEO. Antibiotic resistance was detected in 28 S. aureus strains (80%) and 12 strains (34.3%) were oxacillin and vancomycin-resistant (OVRSA). From the 28 resistant strains, 7 (25%) showed resistance plasmid of 12,000 bp. All strains (n = 35) were sensitive to PAEO and carvacrol, with inhibition zones ranging from 16 to 38 mm and 23 to 42 mm, respectively. The lowest MIC (0.25 mg mL -1 ) and MBC (0.5 mg mL -1 ) values were observed when carvacrol was used against OVRSA. When a 0.5 mg mL -1 concentration of PAEO and carvacrol was used, no viable cells were found on S. aureus biofilm.
Conclusion: The antibacterial effect of carvacrol and PAEO proves to be a possible alternative against planktonic forms and staphylococcal biofilm.
Databáze: MEDLINE
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