Influence of omega-3 fatty acids on bovine luteal cell plasma membrane dynamics.

Autor: Plewes MR; School of Biological Sciences, University of Northern Colorado, Greeley, CO 80639, United States., Burns PD; School of Biological Sciences, University of Northern Colorado, Greeley, CO 80639, United States. Electronic address: Patrick.burns@unco.edu., Hyslop RM; Department of Chemistry and Biochemistry, University of Northern Colorado, Greeley, CO 80639, United States., George Barisas B; Department of Chemistry, Colorado State University, Fort Collins, CO 80523, United States.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. Biomembranes [Biochim Biophys Acta Biomembr] 2017 Dec; Vol. 1859 (12), pp. 2413-2419. Date of Electronic Publication: 2017 Sep 11.
DOI: 10.1016/j.bbamem.2017.09.012
Abstrakt: Fish oil is a rich source of omega-3 fatty acids which disrupt lipid microdomain structure and affect mobility of the prostaglandin F 2α (FP) receptor in bovine luteal cells. The objectives of this study were to determine the effects of individual omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on 1) membrane fatty acid composition, 2) lipid microdomain structure, and 3) lateral mobility of the FP receptor in bovine luteal cells. Ovaries were collected from a local abattoir (n=5/experiment). The corpus luteum was resected and enzymatically digested using collagenase to generate a mixed luteal cell population. In all experiments, luteal cells were treated with 0, 1, 10 or 100μM EPA or DHA for 72h to allow incorporation of fatty acids into membrane lipids. Results from experiment 1 show that culturing luteal cells in the presence of EPA or DHA increased these luteal fatty acids. In experiment 2, both EPA and DHA increased spatial distribution of lipid microdomains in a dose-dependent manner. Single particle tracking results from experiment 3 show that increasing both EPA and DHA concentrations increased micro- and macro-diffusion coefficients, increased domain size, and decreased residence time of FP receptors. Collectively, results from this study demonstrate similar effects of EPA and DHA on lipid microdomain structure and lateral mobility of FP receptors in cultured bovine luteal cells. Moreover, only 10μM of either fatty acid was needed to mimic the effects of fish oil.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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