Protein-Rich Food Ingestion Stimulates Mitochondrial Protein Synthesis in Sedentary Young Adults of Different BMIs.

Autor:	Beals JW; Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., Mackenzie RWA; Department of Life Sciences, University of Roehampton, London SW15 5PU, United Kingdom., van Vliet S; Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., Skinner SK; Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., Pagni BA; Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., Niemiro GM; Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., Ulanov AV; Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., Li Z; Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., Dilger AC; Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., Paluska SA; Department of Family Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., De Lisio M; Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801., Burd NA; Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.; Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Jazyk:	angličtina
Zdroj:	The Journal of clinical endocrinology and metabolism [J Clin Endocrinol Metab] 2017 Sep 01; Vol. 102 (9), pp. 3415-3424.
DOI:	10.1210/jc.2017-00360
Abstrakt:	Context: Excess fat mass may diminish the anabolic potency of protein-rich food ingestion to stimulate muscle protein subfractional synthetic responses. However, the impact of adiposity on mitochondrial protein synthesis (MPS) rates after protein-rich food ingestion has not been thoroughly examined in vivo in humans. Objective: We compared basal and postprandial MPS and markers of muscle inflammation [toll-like receptor 4 (TLR4) and myeloid differentiation primary response protein 88 (MyD88) protein content] in young adults with different body mass indices (BMIs). Methods: Ten normal-weight (NW; BMI = 22.7 ± 0.4 kg/m2), 10 overweight (OW; BMI = 27.1 ± 0.5 kg/m2), and 10 obese (OB; BMI = 35.9 ± 1.3 kg/m2) adults received primed continuous L-[ring-13C6]phenylalanine infusions, blood sampling, and skeletal muscle biopsies before and after the ingestion of 170 g of pork. Results: Pork ingestion increased muscle TLR4 and MyD88 protein content in the OB group (P < 0.05), but not in the NW or OW groups. Basal MPS was similar between groups (P > 0.05). Pork ingestion stimulated MPS (P < 0.001; 0 to 300 minutes) in the NW (2.5- ± 0.6-fold above baseline values), OW (1.7- ± 0.3-fold), and OB groups (2.4- ± 0.5-fold) with no group differences (P > 0.05). Conclusions: Protein-dense food ingestion promotes muscle inflammatory signaling only in OB adults. However, the consumption of a dinner-sized amount of protein strongly stimulated a postprandial MPS response irrespective of BMI. Our data suggest that alterations in postprandial MPS are unlikely to contribute to compromised muscle macronutrient metabolism witnessed with obesity. (Copyright © 2017 Endocrine Society)
Databáze:	MEDLINE
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