Acute doses of caffeine shift nervous system cell expression profiles toward promotion of neuronal projection growth.

Autor: Yu NY; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SE-141 83, Sweden., Bieder A; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SE-141 83, Sweden., Raman A; Department of Medicine (MedH), Karolinska Institutet, Huddinge, SE-141 86, Sweden., Mileti E; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SE-141 83, Sweden., Katayama S; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SE-141 83, Sweden., Einarsdottir E; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SE-141 83, Sweden.; Folkhälsan Institute of Genetics, and Molecular Neurology Research Program, University of Helsinki, Helsinki, 00014, Finland., Fredholm BB; Department of Physiology and Pharmacology, Karolinska Institutet, Solna, SE-171 77, Sweden., Falk A; Department of Neuroscience, Karolinska Institutet, Solna, SE-171 77, Sweden., Tapia-Páez I; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SE-141 83, Sweden.; Department of Medicine/Center for Molecular Medicine, Karolinska University Hospital, Solna, SE-171 76, Sweden., Daub CO; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SE-141 83, Sweden.; Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Tsurumi, Yokohama, Kanagawa, #230-0045, Japan., Kere J; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SE-141 83, Sweden. juha.kere@ki.se.; Folkhälsan Institute of Genetics, and Molecular Neurology Research Program, University of Helsinki, Helsinki, 00014, Finland. juha.kere@ki.se.; Department of Medical and Molecular Genetics, King's College London, London, SE1 9RT, United Kingdom. juha.kere@ki.se.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Sep 13; Vol. 7 (1), pp. 11458. Date of Electronic Publication: 2017 Sep 13.
DOI: 10.1038/s41598-017-11574-6
Abstrakt: Caffeine is a widely consumed psychoactive substance, but little is known about the effects of caffeine stimulation on global gene expression changes in neurons. Here, we conducted gene expression profiling of human neuroepithelial stem cell-derived neurons, stimulated with normal consumption levels of caffeine (3 μM and 10 μM), over a period of 9 h. We found dosage-dependent activation of immediate early genes after 1 h. Neuronal projection development processes were up-regulated and negative regulation of axon extension processes were down-regulated at 3 h. In addition, genes involved in extracellular matrix organization, response for wound healing, and regulation of immune system processes were down-regulated by caffeine at 3 h. This study identified novel genes within the neuronal projection guidance pathways that respond to acute caffeine stimulation and suggests potential mechanisms for the effects of caffeine on neuronal cells.
Databáze: MEDLINE