Melatonin Improves Cognitive Deficits via Restoration of Cholinergic Dysfunction in a Mouse Model of Scopolamine-Induced Amnesia.

Autor: Chen BH; Department of Histology and Embryology, Institute of Neuroscience , Wenzhou Medical University , Wenzhou , Zhejiang 325035 , P.R. China., Park JH; Department of Biomedical Science and Research Institute for Bioscience and Biotechnology , Hallym University , Chuncheon 24252 , South Korea., Kim DW; Department of Biochemistry and Molecular Biology, and Research Institute of Oral Sciences, College of Dentistry , Kangnung-Wonju National University , Gangneung 25457 , South Korea., Park J; Department of Biomedical Science and Research Institute for Bioscience and Biotechnology , Hallym University , Chuncheon 24252 , South Korea., Choi SY; Department of Biomedical Science and Research Institute for Bioscience and Biotechnology , Hallym University , Chuncheon 24252 , South Korea., Kim IH; Department of Neurobiology , School of Medicine, Kangwon National University , Chuncheon 24341 , South Korea., Cho JH; Department of Neurobiology , School of Medicine, Kangwon National University , Chuncheon 24341 , South Korea., Lee TK; Department of Neurobiology , School of Medicine, Kangwon National University , Chuncheon 24341 , South Korea., Lee JC; Department of Neurobiology , School of Medicine, Kangwon National University , Chuncheon 24341 , South Korea., Lee CH; Department of Pharmacy, College of Pharmacy , Dankook University , Cheonan 31116 , South Korea., Hwang IK; Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science , Seoul National University , Seoul 08826 , South Korea., Kim YM; Department of Molecular and Cellular Biochemistry, School of Medicine , Kangwon National University , Chuncheon , South Korea., Yan BC; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou 225001 , People's Republic of China., Kang IJ; Department of Food Science and Nutrition , Hallym University , Chuncheon 24252 , South Korea., Shin BN; Department of Physiology, College of Medicine, and Institute of Neurodegeneration and Neuroregeneration , Hallym University , Chuncheon 24252 , South Korea., Lee YL; Department of Physiology, College of Medicine, and Institute of Neurodegeneration and Neuroregeneration , Hallym University , Chuncheon 24252 , South Korea., Shin MC; Department of Emergency Medicine, School of Medicine , Kangwon National University , Chuncheon 24341 , South Korea., Cho JH; Department of Emergency Medicine, School of Medicine , Kangwon National University , Chuncheon 24341 , South Korea., Lee YJ; Department of Emergency Medicine, Seoul Hospital, College of Medicine , Sooncheonhyang University , Seoul 04401 , South Korea., Jeon YH; Department of Radiology, School of Medicine , Kangwon National University , Chuncheon 24289 , South Korea., Won MH; Department of Neurobiology , School of Medicine, Kangwon National University , Chuncheon 24341 , South Korea., Ahn JH; Department of Biomedical Science and Research Institute for Bioscience and Biotechnology , Hallym University , Chuncheon 24252 , South Korea.
Jazyk: angličtina
Zdroj: ACS chemical neuroscience [ACS Chem Neurosci] 2018 Aug 15; Vol. 9 (8), pp. 2016-2024. Date of Electronic Publication: 2017 Sep 27.
DOI: 10.1021/acschemneuro.7b00278
Abstrakt: Melatonin is known to improve cognitive deficits, and its functions have been studied in various disease models, including Alzheimer's disease. In this study, we investigated effects of melatonin on cognition and the cholinergic system of the septum and hippocampus in a mouse model of scopolamine-induced amnesia. Scopolamine (1 mg/kg) and melatonin (10 mg/kg) were administered intraperitoneally to mice for 2 and 4 weeks. The Morris water maze and passive avoidance tests revealed that both treatments of scopolamine significantly impaired spatial learning and memory; however, 2- and 4-week melatonin treatments significantly improved spatial learning and memory. In addition, scopolamine treatments significantly decreased protein levels and immunoreactivities of choline acetyltransferase (ChAT), high-affinity choline transporter (CHT), vesicular acetylcholine transporter (VAChT), and muscarinic acetylcholine receptor M1 (M1R) in the septum and hippocampus. However, the treatments with melatonin resulted in increased ChAT-, CHT-, VAChT-, and M1R-immunoreactivities and their protein levels in the septum and hippocampus. Our results demonstrate that melatonin treatment is effective in improving the cognitive deficits via restoration of the cholinergic system in the septum and hippocampus of a mouse model of scopolamine-induced amnesia.
Databáze: MEDLINE