Total Leishmania antigens with Poly(I:C) induce Th1 protective response.

Autor: Sanchez MV; Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), Centro Científico y Tecnológico de Mendoza (CCT-Mendoza), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina., Eliçabe RJ; Instituto Multidisciplinario de Investigaciones Biológicas San Luis (IMIBIO-SL), Centro Científico y Tecnológico de San Luis (CCT-San Luis), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad de San Luis, San Luis, Argentina., Di Genaro MS; Instituto Multidisciplinario de Investigaciones Biológicas San Luis (IMIBIO-SL), Centro Científico y Tecnológico de San Luis (CCT-San Luis), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad de San Luis, San Luis, Argentina., Germanó MJ; Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), Centro Científico y Tecnológico de Mendoza (CCT-Mendoza), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina., Gea S; Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Centro Científico y Tecnológico de Córdoba (CCT-Córdoba), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Córdoba, Argentina., García Bustos MF; Instituto de Patología Experimental (IPE), Centro Científico y Tecnológico de Salta (CCT-Salta), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Salta, Argentina., Salomón MC; Area de Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo (UNCuyo), UNCUYO Centro Universitario (M5502JMA), Mendoza, Argentina., Scodeller EA; Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), Centro Científico y Tecnológico de Mendoza (CCT-Mendoza), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina., Cargnelutti DE; Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), Centro Científico y Tecnológico de Mendoza (CCT-Mendoza), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina.; Area de Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo (UNCuyo), UNCUYO Centro Universitario (M5502JMA), Mendoza, Argentina.
Jazyk: angličtina
Zdroj: Parasite immunology [Parasite Immunol] 2017 Nov; Vol. 39 (11). Date of Electronic Publication: 2017 Oct 09.
DOI: 10.1111/pim.12491
Abstrakt: Our proposal was to develop a vaccine based on total Leishmania antigens (TLA) adjuvanted with polyinosinic-polycytidylic acid [Poly(I:C)] able to induce a Th1 response which can provide protection against Leishmania infection. Mice were vaccinated with two doses of TLA-Poly(I:C) administered by subcutaneous route at 3-week interval. Humoral and cellular immune responses induced by the immunization were measured. The protective efficacy of the vaccine was evaluated by challenging mice with infective promastigotes of Leishmania (Leishmania) amazonensis into the footpad. Mice vaccinated with TLA-Poly(I:C) showed a high anti-Leishmania IgG titre, as well as increased IgG1 and IgG2a subclass titres compared with mice vaccinated with the TLA alone. The high IgG2a indicated a Th1 bias response induced by the TLA-Poly(I:C) immunization. Accordingly, the cellular immune response elicited by the formulation was characterized by an increased production of IFN-γ and no significant production of IL-4. The TLA-Poly(I:C) immunization elicited good protection, which was associated with decreased footpad swelling, a lower parasite load and a reduced histopathological alteration in the footpad. Our findings demonstrate a promising vaccine against cutaneous leishmaniasis that is relatively economic and easy to develop and which should be taken into account for preventing leishmaniasis in developing countries.
(© 2017 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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