| Autor: |
Tikhonov MV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.; me@mtih.me., Maksimenko OG; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia., Georgiev PG; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia., Korobko IV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia. |
| Jazyk: |
ruština |
| Zdroj: |
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2017 Jul-Aug; Vol. 51 (4), pp. 671-676. |
| DOI: |
10.7868/S0026898417040176 |
| Abstrakt: |
Introns can frequently enhance transgene expression, and sometimes they are absolutely substantial. Based on an analysis of murine genes, in which mRNA does not have alternative splicing, a universal design of the efficiently spliced artificial introns of small sizes has been proposed. These introns are shown to be efficiently spliced in CHO cells from hamster ovaries. The proposed strategy can be used to include introns in cDNA, which would elevate the production of recombinant proteins in cell culture, as well as in transgenic animals. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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