| Autor: |
Bonamigo T; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil., Campos JF; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil., Oliveira AS; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil., Torquato HFV; Faculty of Pharmacy, Braz Cubas University, Francisco Rodrigues Filho Avenue, Mogi das Cruzes, SP, Brazil.; Department of Biochemistry, Federal University of São Paulo, R. Três de Maio 100, São Paulo, SP, Brazil., Balestieri JBP; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil., Cardoso CAL; Course of Chemistry, State University of Mato Grosso do Sul, Dourados, MS, Brazil., Paredes-Gamero EJ; Course of Chemistry, State University of Mato Grosso do Sul, Dourados, MS, Brazil.; Center for Interdisciplinary Research Biochemistry, University of Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida Souza, Mogi das Cruzes, SP, Brazil., de Picoli Souza K; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil., Dos Santos EL; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil. |
| Abstrakt: |
Propolis is a complex bioactive mixture produced by bees, known to have different biological activities, especially in countries where there is a rich biodiversity of plant species. The objective of this study was to determine the chemical composition and evaluate the antioxidant and cytotoxic properties of Brazilian propolis from the species Plebeia droryana and Apis mellifera found in Mato Grosso do Sul, Brazil. In the ethanolic extracts of P. droryana propolis (ExEP-P) and A. mellifera (ExEP-A) acids, phenolic compounds, terpenes and tocopherol were identified as major compounds. Both extracts presented antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, the maximum activities being 500 μg/mL (ExEP-P) and 300 μg/mL (ExEP-A). However, only ExEP-A was able to inhibit lipid peroxidation induced by the oxidizing agent 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), which inhibited oxidative hemolysis and reduced the levels of malondialdehyde (MDA) in human erythrocytes for 4 h of incubation. The extracts also reduced the cell viability of the K562 erythroleukemia tumour line, with a predominance of necrotic death. Thus, it is concluded that the propolis produced by P. droryana and A. mellifera contain important compounds capable of minimizing the action of oxidizing substances in the organism and reducing the viability of erythroleukemia cells. |
