Molecular characterization of circulating Foot and mouth disease virus (FMDV) serotype O topotype EA-3 and serotype A (African topotype) genotype IV in Egypt, 2016.

Autor: Soltan MA; Department of Veterinary Medicine, Infectious Diseases Division, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt. Electronic address: msoltan@vet.suez.edu.eg., Negmaldin AH; Ismailia, Egypt., El-Diasty MM; Mansoura Provincial Laboratory, Animal Health Research Institute, Mansoura, Egypt., Mansour SMG; Department of Virology, Faculty Veterinary Medicine, Zagazig University, Zagazig, Egypt., Elbadry MA; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610, USA., Wilkes RP; Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, 43 Brighton Road, Tifton, GA, USA.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2017 Sep; Vol. 208, pp. 89-93. Date of Electronic Publication: 2017 Jul 18.
DOI: 10.1016/j.vetmic.2017.07.018
Abstrakt: In January-April 2016, cattle and buffalo farm owners and veterinarians reported clinical signs suggestive of foot and mouth disease virus (FMDV) outbreaks among non-vaccinated cattle and buffalo herds in Egypt. The clinical disease observed was either mild (small oral lesions and speedy recovery) or severe (extensive oral lesions and/or mortalities), and the form of the disease (either mild or severe) segregated by farm. This study aimed to confirm the presence of FMDV and to characterize the circulating strains associated with the outbreaks. Vesicular epithelia were collected from 41 animals representing 15 affected cattle and buffalo farms in five governorates (Behira, Cairo, Daqahlia, Giza and Ismailia), and tested by real time (rt) RT-PCR. Consequently, 92% (38/41) of examined samples were positive. Furthermore, the VP1 coding region of 60% (23/38) of positive specimens were amplified by RT-PCR and sequenced. The phylogenetic analysis identified two distinct strains characterized as serotype O topotype EA-3 and serotype A (African topotype) of genotype IV in the severe and mild disease forms, respectively. The newly identified strains clustered in distinct clades in the phylogenetic trees, indicating the likelihood of new incursions into Egypt. Those strains were most closely related to previously described Sudanese strains.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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