Osteoinductive effects of preoperative dexamethasone in human dental pulp stem cells primary culture.

Autor:	Moretti RDC; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal, de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal, de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil., Duailibi MT; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal, de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil.; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal, de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil., Martins PO; Oral & Maxillofacial Surgery Department, Hospital Ipiranga, São Paulo, Brazil.; Oral & Maxillofacial Surgery Department, Hospital Ipiranga, São Paulo, Brazil., Dos Santos JA; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal, de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal, de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil., Duailibi SE; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal, de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil.; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal, de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de São Paulo- Escola Paulista de Medicina, São Paulo, Brazil.; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Future science OA [Future Sci OA] 2017 Apr 03; Vol. 3 (3), pp. FSO184. Date of Electronic Publication: 2017 Apr 03 (Print Publication: 2017).
DOI:	10.4155/fsoa-2016-0083
Abstrakt:	Aim: The use of dexamethasone (DEX) in mesenchymal cell culture induces osteoblastic differentiation and, consequently, formation of mineralized tissues. Tissue engineering proposes the development of therapeutic strategies aimed at structural and functional regeneration of biological tissues. In this sense, cell characterization in vitro is critical to ensure the development of such techniques. Our objective was to evaluate the osteoinductive effect of DEX administered as a preoperative medication in primary cell culture of human dental pulp stem cell. Methodology: Cells from the third molar pulp were divided into two experimental groups, each with two preoperative medication protocols used in dental practice and differentiated by the intake of DEX in one of them. The assessment of proliferation, differentiation and viability through trypan blue, methylthiazol tetrazolium, and von Kossa and alizarin red assays, respectively, were held within fixed intervals: 7, 14, 21 and 28 days. Conclusion: This study has shown that DEX may influence in vitro human dental pulp stem cell behavior. Competing Interests: Financial & competing interests disclosure The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. No writing assistance was utilized in the production of this manuscript.
Databáze:	MEDLINE
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