Bioluminescent enzyme inhibition-based assay to predict the potential toxicity of carbon nanomaterials.

Autor: Esimbekova EN; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia. Electronic address: esimbekova@yandex.ru., Nemtseva EV; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia., Bezrukikh AE; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia., Jukova GV; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia., Lisitsa AE; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia., Lonshakova-Mukina VI; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia., Rimatskaya NV; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia., Sutormin OS; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia., Kratasyuk VA; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2017 Dec; Vol. 45 (Pt 1), pp. 128-133. Date of Electronic Publication: 2017 Sep 04.
DOI: 10.1016/j.tiv.2017.08.022
Abstrakt: A bioluminescent enzyme inhibition-based assay was applied to predict the potential toxicity of carbon nanomaterials (CNM) presented by single- and multi-walled nanotubes (SWCNT and MWCNT) and aqueous solutions of hydrated fullerene С 60 (C 60 HyFn). This assay specifically detects the influence of substances on parameters of the soluble or immobilised coupled enzyme system of luminescent bacteria: NAD(P)Н:FMN-oxidoreductase+luciferase (Red+Luc). A protocol based on the optical properties of CNM for correcting the results of the bioluminescent assay was also developed. It was shown that the inhibitory activity of CNM on Red+Luc decreased in the following order: MWCNT>SWCNT>C 60 HyFn. The soluble enzyme system Red+Luc had high sensitivity to MWCNT and SWCNT, with values of the inhibition parameter IC 50 equal to 0.012 and 0.16mg/L, respectively. The immobilised enzyme system was more vulnerable to C 60 HyFn than its soluble form, with an IC 50 equal to 1.4mg/L. Due to its technical simplicity, rapid response time and high sensitivity, this bioluminescent method has the potential to be developed as a general enzyme inhibition-based assay for a wide variety of nanomaterials.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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