Measuring Tau-microtubule affinity through cosedimentation assays.
| Autor: | Alberico EO; University of Notre Dame, Notre Dame, IN, United States., Duan AR; University of Notre Dame, Notre Dame, IN, United States., Goodson HV; University of Notre Dame, Notre Dame, IN, United States. Electronic address: hgoodson@nd.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in cell biology [Methods Cell Biol] 2017; Vol. 141, pp. 115-134. |
| DOI: | 10.1016/bs.mcb.2017.06.015 |
| Abstrakt: | Microtubule cosedimentation assays have long been used to study the affinity of interactions between Tau protein and microtubules. While these assays are very useful for characterizing and comparing the effects of alterations to either Tau or the microtubule filaments, they can also be problematic. We provide a set of straightforward instructions for performing these assays and point out a number of challenges and pitfalls that can complicate their interpretation. (© 2017 Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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