Slc7a11 (xCT) protein expression is not altered in the depressed brain and system xc- deficiency does not affect depression-associated behaviour in the corticosterone mouse model.

Autor: Demuyser T; a Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information , Center for Neurosciences (C4N), Vrije Universiteit Brussel , Brussels , Belgium., Deneyer L; b Department of Pharmaceutical Biotechnology and Molecular Biology , C4N, Vrije Universiteit Brussel , Brussels , Belgium., Bentea E; b Department of Pharmaceutical Biotechnology and Molecular Biology , C4N, Vrije Universiteit Brussel , Brussels , Belgium., Albertini G; a Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information , Center for Neurosciences (C4N), Vrije Universiteit Brussel , Brussels , Belgium., Femenia T; c Department of Neuroscience , Karolinska Institutet , Stockholm , Sweden., Walrave L; a Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information , Center for Neurosciences (C4N), Vrije Universiteit Brussel , Brussels , Belgium., Sato H; d Laboratory of Biochemistry and Molecular Biology, Department of Medical Technology , Niigata University , Niigata , Japan., Danbolt NC; e Department of Molecular Medicine , Institute of Basic Medical Sciences, University of Oslo , Oslo , Norway., De Bundel D; a Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information , Center for Neurosciences (C4N), Vrije Universiteit Brussel , Brussels , Belgium., Michotte A; f Experimental Pathology and Neuropathology , University Hospital Brussels, Vrije Universiteit Brussel , Brussels , Belgium., Lindskog M; c Department of Neuroscience , Karolinska Institutet , Stockholm , Sweden., Massie A; b Department of Pharmaceutical Biotechnology and Molecular Biology , C4N, Vrije Universiteit Brussel , Brussels , Belgium., Smolders I; a Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information , Center for Neurosciences (C4N), Vrije Universiteit Brussel , Brussels , Belgium.
Jazyk: angličtina
Zdroj: The world journal of biological psychiatry : the official journal of the World Federation of Societies of Biological Psychiatry [World J Biol Psychiatry] 2019 Jun; Vol. 20 (5), pp. 381-392. Date of Electronic Publication: 2017 Sep 27.
DOI: 10.1080/15622975.2017.1371332
Abstrakt: Objectives: The cystine/glutamate antiporter (system xc-) is believed to contribute to nonvesicular glutamate release from glial cells in various brain areas. Although recent investigations implicate system xc- in mood disorders, unambiguous evidence has not yet been established. Therefore, we evaluated the possible role of system xc- in the depressive state. Methods: We conducted a protein expression analysis of the specific subunit of system xc- (xCT) in brain regions of the corticosterone mouse model, Flinders Sensitive Line rat model and post-mortem tissue of depressed patients. We next subjected system xc- deficient mice to the corticosterone model and analysed their behaviour in several tests. Lastly, we subjected additional cohorts of xCT-deficient and wild-type mice to N -acetylcysteine treatment to unveil whether the previously reported antidepressant-like effects are dependent upon system xc-. Results: We did not detect any changes in xCT expression levels in the animal models or patients compared to proper controls. Furthermore, loss of system xc- had no effect on depression- and anxiety-like behaviour. Finally, the antidepressant-like effects of N- acetylcysteine are not mediated via system xc-. Conclusions: xCT protein expression is not altered in the depressed brain and system xc- deficiency does not affect depression-associated behaviour in the corticosterone mouse model.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje