| Autor: |
Sierra EM; Department of Technology, São Paulo State University, Jaboticabal, São Paulo State, Brazil.; Universidad Simón Bolívar, Barranquilla, Colombia., Pereira MR; Institute for Research in Bioenergy (IPBEN), Jaboticabal, São Paulo State, Brazil., Maester TC; Institute for Research in Bioenergy (IPBEN), Jaboticabal, São Paulo State, Brazil., Gomes-Pepe ES; Department of Technology, São Paulo State University, Jaboticabal, São Paulo State, Brazil.; Institute for Research in Bioenergy (IPBEN), Jaboticabal, São Paulo State, Brazil., Mendoza ER; Department of Technology, São Paulo State University, Jaboticabal, São Paulo State, Brazil., Lemos EGM; Department of Technology, São Paulo State University, Jaboticabal, São Paulo State, Brazil. egerle@fcav.unesp.br.; Institute for Research in Bioenergy (IPBEN), Jaboticabal, São Paulo State, Brazil. egerle@fcav.unesp.br.; Av. Prof. Paulo Donato Castellane, s/n. Jaboticabal, Post code 14884-900, São Paulo State, Brazil. egerle@fcav.unesp.br. |
| Abstrakt: |
The aminopeptidase gene from Mesorhizobium SEMIA3007 was cloned and overexpressed in Escherichia coli. The enzyme called MesoAmp exhibited optimum activity at pH 8.5 and 45 °C and was strongly activated by Co 2+ and Mn 2+ . Under these reaction conditions, the enzyme displayed K m and k cat values of 0.2364 ± 0.018 mM and 712.1 ± 88.12 s -1 , respectively. Additionally, the enzyme showed remarkable stability in organic solvents and was active at high concentrations of NaCl, suggesting that the enzyme might be suitable for use in biotechnology. MesoAmp is responsible for 40% of the organism's aminopeptidase activity. However, the enzyme's absence does not affect bacterial growth in synthetic broth, although it interfered with biofilm synthesis and osmoregulation. To the best of our knowledge, this report describes the first detailed characterization of aminopeptidase from Mesorhizobium and suggests its importance in biofilm formation and osmotic stress tolerance. In summary, this work lays the foundation for potential biotechnological applications and/or the development of environmentally friendly technologies and describes the first solvent- and halo-tolerant aminopeptidases identified from the Mesorhizobium genus and its importance in bacterial metabolism. |
