Pf16 and phiPMW: Expanding the realm of Pseudomonas putida bacteriophages.

Autor: Magill DJ; Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast, Northern Ireland., Krylov VN; Department of Microbiology, Laboratory for Genetics of Bacteriophages, I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia., Shaburova OV; Department of Microbiology, Laboratory for Genetics of Bacteriophages, I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia., McGrath JW; Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast, Northern Ireland., Allen CCR; Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast, Northern Ireland., Quinn JP; Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast, Northern Ireland., Kulakov LA; Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast, Northern Ireland.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 Sep 06; Vol. 12 (9), pp. e0184307. Date of Electronic Publication: 2017 Sep 06 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0184307
Abstrakt: We present the analysis of two novel Pseudomonas putida phages, pf16 and phiPMW. Pf16 represents a peripherally related T4-like phage, and is the first of its kind infecting a Pseudomonad, with evidence suggesting cyanophage origins. Extensive divergence has resulted in pf16 occupying a newly defined clade designated as the pf16-related phages, lying at the interface of the Schizo T-Evens and Exo T-Evens. Recombination with an ancestor of the P. putida phage AF is likely responsible for the tropism of this phage. phiPMW represents a completely novel Pseudomonas phage with a genome containing substantial genetic novelty through its many hypothetical proteins. Evidence suggests that this phage has been extensively shaped through gene transfer events and vertical evolution. Phylogenetics shows that this phage has an evolutionary history involving FelixO1-related viruses but is in itself highly distinct from this group.
Databáze: MEDLINE
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