Glutamine and citrulline concentrations reflect nitric oxide synthesis in the human nervous system.

Autor: Pérez-Neri I; Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Tlalpan, Ciudad de México, México., Ramírez-Bermúdez J; Departamento de Psiquiatría, Instituto Nacional de Neurología y Neurocirugía, Tlalpan, Ciudad de México, México., Ojeda-López C; Departamento de Psiquiatría, Instituto Nacional de Neurología y Neurocirugía, Tlalpan, Ciudad de México, México., Montes S; Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Tlalpan, Ciudad de México, México., Soto-Hernández JL; Departamento de Infectología, Instituto Nacional de Neurología y Neurocirugía, Tlalpan, Ciudad de México, México., Ríos C; Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Tlalpan, Ciudad de México, México. Electronic address: crios@correo.xoc.uam.mx.
Jazyk: English; Spanish; Castilian
Zdroj: Neurologia (Barcelona, Spain) [Neurologia (Engl Ed)] 2020 Mar; Vol. 35 (2), pp. 96-104. Date of Electronic Publication: 2017 Sep 01.
DOI: 10.1016/j.nrl.2017.07.013
Abstrakt: Introduction: Although citrulline is produced by nitric oxide (NO) synthase upon activation of the NMDA glutamate receptor, nitrite and nitrate (NO x ) concentration is considered the best marker of NO synthesis, as citrulline is also metabolised by other enzymes. This study analyses the correlation between human cerebrospinal fluid NO x and citrulline concentrations in order to determine the extent to which citrulline reflects NO synthesis and glutamatergic neurotransmission.
Methods: Participants were patients with acute neurological diseases undergoing lumbar puncture (n=240). NO x and amino acid concentrations were determined by HPLC.
Results: NO x concentrations did not vary significantly where infection (p=0,110) or inflammation (p=0,349) were present. Multiple regression analysis showed that NO x concentration was correlated with glutamine (r=-0,319, p<0,001) and citrulline concentrations (r=0,293, p=0,005) but not with the citrulline/arginine ratio (r=-0,160, p=0,173). ANCOVA confirmed that NO x concentration was correlated with citrulline concentration (F=7,6, p=0,007) but not with the citrulline/arginine ratio (F=2,2, p=0,136), or presence of infection (F=1,8, p=0,173) or inflammation (F=1,4, p=0,227). No association was found between NO x and arginine or glutamate concentrations.
Conclusion: The results suggest that CSF citrulline concentration reflects NO x synthesis to some extent, despite the contribution of other metabolic pathways. In addition, this study shows that glutamine is an important modulator of NO synthase activity, and that arginine and glutamate are not correlated with NO x .
(Copyright © 2017 Sociedad Española de Neurología. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze: MEDLINE
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