Comparative in vitro study of photodynamic activity of hypericin and hypericinates in MCF-7 cells.

Autor: de Andrade GP; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, Av. dos Estados, 5001, Bairro Bangú, Santo André, SP, Brazil., Manieri TM; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, Av. dos Estados, 5001, Bairro Bangú, Santo André, SP, Brazil., Nunes EA; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, Av. dos Estados, 5001, Bairro Bangú, Santo André, SP, Brazil., Viana GM; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, Av. dos Estados, 5001, Bairro Bangú, Santo André, SP, Brazil., Cerchiaro G; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, Av. dos Estados, 5001, Bairro Bangú, Santo André, SP, Brazil., Ribeiro AO; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, Av. dos Estados, 5001, Bairro Bangú, Santo André, SP, Brazil. Electronic address: anderson.ribeiro@ufabc.edu.br.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2017 Oct; Vol. 175, pp. 89-98. Date of Electronic Publication: 2017 Aug 24.
DOI: 10.1016/j.jphotobiol.2017.08.025
Abstrakt: In this work we present a comparative in vitro study of photodynamic activity between hypericin (HYP) and some hypericinates (hypericin ionic pair with lysine or N-methylglucamine) in human mammary adenocarcinoma cells (MCF-7). The toxicity and phototoxicity of hypericin and hypericinates were compared, as well as their cellular uptake and localization and mutagenic, genotoxic and clonogenic capacity. Our results demonstrate that different cationic moieties promote differences in the hypericinate solubility in a biological environment, and can influence the cellular localization and the phototoxicity of the photosensitizer. It was verified that hypericinates have better efficiency to generate singlet oxygen than HYP, and a lower aggregation in biological medium. In vitro assays have shown that HYP and the hypericinates are able to permeate the MCF-7 cell membrane and accumulated in organelles near the nucleus. The difference in location, however, was not determinant to the cell death mechanism, and a higher prevalence of apoptosis for all studied compounds occurred. The photodynamic studies indicated that hypericinates were more effective than HYP and were able to inhibit the formation of cellular colonies, suggesting a possible ability to prevent the recurrence of tumors. It also appears that all compounds have relative safety for mutagenicity and genotoxicity, which opens up a further safe route for application in in vivo studies.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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