Structural basis for antibacterial peptide self-immunity by the bacterial ABC transporter McjD.

Autor: Bountra K; Department of Life Sciences, Imperial College London, London, UK.; Rutherford Appleton Laboratory, Research Complex at Harwell, Oxfordshire, UK., Hagelueken G; Institute for Physical and Theoretical Chemistry, University of Bonn, Bonn, Germany., Choudhury HG; Department of Life Sciences, Imperial College London, London, UK.; Rutherford Appleton Laboratory, Research Complex at Harwell, Oxfordshire, UK., Corradi V; Centre for Molecular Simulation and Department of Biological Sciences, University of Calgary, Calgary, AB, Canada., El Omari K; Rutherford Appleton Laboratory, Research Complex at Harwell, Oxfordshire, UK.; Diamond Light Source, Oxfordshire, UK., Wagner A; Rutherford Appleton Laboratory, Research Complex at Harwell, Oxfordshire, UK.; Diamond Light Source, Oxfordshire, UK., Mathavan I; Department of Life Sciences, Imperial College London, London, UK.; Rutherford Appleton Laboratory, Research Complex at Harwell, Oxfordshire, UK., Zirah S; Communication Molecules and Adaptation of Microorganisms Laboratory (MCAM, UMR 7245 CNRS-MNHN), Muséum National d'Histoire Naturelle, Centre National de la Recherche Scientifique, Sorbonne Universités, Paris, France., Yuan Wahlgren W; Department of Life Sciences, Imperial College London, London, UK.; Rutherford Appleton Laboratory, Research Complex at Harwell, Oxfordshire, UK.; Chemistry & Molecular Biology, University of Gothenburg, Göteborg, Sweden., Tieleman DP; Centre for Molecular Simulation and Department of Biological Sciences, University of Calgary, Calgary, AB, Canada., Schiemann O; Institute for Physical and Theoretical Chemistry, University of Bonn, Bonn, Germany., Rebuffat S; Communication Molecules and Adaptation of Microorganisms Laboratory (MCAM, UMR 7245 CNRS-MNHN), Muséum National d'Histoire Naturelle, Centre National de la Recherche Scientifique, Sorbonne Universités, Paris, France., Beis K; Department of Life Sciences, Imperial College London, London, UK kbeis@imperial.ac.uk.; Rutherford Appleton Laboratory, Research Complex at Harwell, Oxfordshire, UK.
Jazyk: angličtina
Zdroj: The EMBO journal [EMBO J] 2017 Oct 16; Vol. 36 (20), pp. 3062-3079. Date of Electronic Publication: 2017 Sep 01.
DOI: 10.15252/embj.201797278
Abstrakt: Certain pathogenic bacteria produce and release toxic peptides to ensure either nutrient availability or evasion from the immune system. These peptides are also toxic to the producing bacteria that utilize dedicated ABC transporters to provide self-immunity. The ABC transporter McjD exports the antibacterial peptide MccJ25 in Escherichia coli Our previously determined McjD structure provided some mechanistic insights into antibacterial peptide efflux. In this study, we have determined its structure in a novel conformation, apo inward-occluded and a new nucleotide-bound state, high-energy outward-occluded intermediate state, with a defined ligand binding cavity. Predictive cysteine cross-linking in E. coli membranes and PELDOR measurements along the transport cycle indicate that McjD does not undergo major conformational changes as previously proposed for multi-drug ABC exporters. Combined with transport assays and molecular dynamics simulations, we propose a novel mechanism for toxic peptide ABC exporters that only requires the transient opening of the cavity for release of the peptide. We propose that shielding of the cavity ensures that the transporter is available to export the newly synthesized peptides, preventing toxic-level build-up.
(© 2017 The Authors. Published under the terms of the CC BY 4.0 license.)
Databáze: MEDLINE
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