Targeting Type IV Secretion System Proteins to Combat Multidrug-Resistant Gram-positive Pathogens.

Autor:	Laverde D; Division of Pediatric Infectious Diseases, Dr. von Hauner Children's Hospital, Ludwig Maximilians University, Munich., Probst I; Division of Infectious Diseases, Department of Medicine, University Medical Center Freiburg.; Faculty of Biology, Microbiology, Albert Ludwigs University Freiburg., Romero-Saavedra F; Division of Pediatric Infectious Diseases, Dr. von Hauner Children's Hospital, Ludwig Maximilians University, Munich., Kropec A; Division of Infectious Diseases, Department of Medicine, University Medical Center Freiburg., Wobser D; Division of Infectious Diseases, Department of Medicine, University Medical Center Freiburg., Keller W; Institute of Molecular Biosciences, NAWI Graz, University of Graz, Austria., Grohmann E; Division of Infectious Diseases, Department of Medicine, University Medical Center Freiburg.; Microbiology, Faculty of Life Sciences and Technology, Beuth University of Applied Sciences Berlin, Germany., Huebner J; Division of Pediatric Infectious Diseases, Dr. von Hauner Children's Hospital, Ludwig Maximilians University, Munich.; Division of Infectious Diseases, Department of Medicine, University Medical Center Freiburg.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2017 Jun 15; Vol. 215 (12), pp. 1836-1845.
DOI:	10.1093/infdis/jix227
Abstrakt:	For many gram-positive pathogens, conjugative plasmid transfer is an important means of spreading antibiotic resistance . Therefore, the search for alternative treatments to fight and prevent infections caused by these bacteria has become of major interest. In the present study, we evaluated the protein TraM, from the conjugative plasmid pIP501, as a potential vaccine candidate. Anti-TraM antiserum mediated in vitro opsonophagocytic killing of the strain harboring the pIP501 plasmid and also proved to be cross-reactive against other clinically relevant enterococcal and staphylococcal strains. Specificity of antibodies toward TraM was confirmed by results of an opsonophagocytic inhibition assay and Western blot. In addition, conjugative transfer experiments proved that TraM is essential for the transfer of pIP501. Finally, immunization with either TraM or anti-TraM antiserum reduced significantly the colony counts in mice livers, demonstrating that TraM is a promising vaccine candidate against enterococci and other gram-positive pathogens. (© The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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