Enzymatic Synthesis of Mannosyl-cyclodextrin by α-Mannosidase from Jack Bean.

Autor: Hamayasu K; a Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd. , 13-46 Daikoku-cho, Tsurumi-ku, Yokohama 230 , Japan., Hara K; a Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd. , 13-46 Daikoku-cho, Tsurumi-ku, Yokohama 230 , Japan., Fujita K; a Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd. , 13-46 Daikoku-cho, Tsurumi-ku, Yokohama 230 , Japan., Kondo Y; a Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd. , 13-46 Daikoku-cho, Tsurumi-ku, Yokohama 230 , Japan., Hashimoto H; a Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd. , 13-46 Daikoku-cho, Tsurumi-ku, Yokohama 230 , Japan., Tanimoto T; a Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd. , 13-46 Daikoku-cho, Tsurumi-ku, Yokohama 230 , Japan.; b School of Pharmaceutical Sciences, Mukogawa Women's University , 11-68 Koshien Kyuban-cho, Nishinomiya 663 , Japan., Koizumi K; a Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd. , 13-46 Daikoku-cho, Tsurumi-ku, Yokohama 230 , Japan.; b School of Pharmaceutical Sciences, Mukogawa Women's University , 11-68 Koshien Kyuban-cho, Nishinomiya 663 , Japan., Nakano H; a Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd. , 13-46 Daikoku-cho, Tsurumi-ku, Yokohama 230 , Japan.; c Osaka Municipal Technical Research Institute , 1-6-50 Morinomiya, Jyoto-ku, Osaka 536 , Japan., Kitahata S; a Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd. , 13-46 Daikoku-cho, Tsurumi-ku, Yokohama 230 , Japan.; c Osaka Municipal Technical Research Institute , 1-6-50 Morinomiya, Jyoto-ku, Osaka 536 , Japan.
Jazyk: angličtina
Zdroj: Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 1997 Jan; Vol. 61 (5), pp. 825-829.
DOI: 10.1271/bbb.61.825
Abstrakt: Mannosylated derivatives of cyclodextrins (CDs), mannosyl-α, β, and γCD were synthesized from a mixture of mannose and α, β, and γCD by the reverse action of α-mannosidase from jack bean, respectively. Their structures were analyzed by FAB-MS and 13 C-NMR spectroscopies, and they were identified as 6-O-α-d-mannosyl-α, β, and γCD. The optimum conditions for the production of 6-O-α-d-mannosyl-αCD by α-mannosidase were examined. Optimum pH and temperature were pH 4.5 and 60°C, respectively. Yield of mannosyl-αCD increased with increasing mannose concentration and reached more than 35% (mol/mol) at the concentration of 2 m mannose and 0.4 m αCD.
Databáze: MEDLINE
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