miR-92a regulates coelomocytes apoptosis in sea cucumber Apostichopus japonicus via targeting Aj14-3-3ζ in vivo.

Autor: Lv M; School of Marine Sciences, Ningbo University, PR China., Chen H; School of Marine Sciences, Ningbo University, PR China., Shao Y; School of Marine Sciences, Ningbo University, PR China., Li C; School of Marine Sciences, Ningbo University, PR China. Electronic address: lichenghua@nbu.edu.cn., Zhang W; School of Marine Sciences, Ningbo University, PR China., Zhao X; School of Marine Sciences, Ningbo University, PR China., Jin C; School of Marine Sciences, Ningbo University, PR China., Xiong J; School of Marine Sciences, Ningbo University, PR China.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2017 Oct; Vol. 69, pp. 211-217. Date of Electronic Publication: 2017 Aug 30.
DOI: 10.1016/j.fsi.2017.08.033
Abstrakt: miR-92a, a well-documented oncogene, was previously found to be differentially expressed in diseased sea cucumber Apostichopus japonicus by high-throughput sequencing. In this study, we identified Aj14-3-3ζ as a novel target of miR-92a in this species and investigated their regulatory roles in vivo. The negative expression profiles between miR-92a and Aj14-3-3ζ protein were detected in both LPS-exposed primary coelomocytes and Vibrio splendidus-challenged sea cucumbers. Over-expression of miR-92a by injection of miR-92a agomir significantly depressed the mRNA and protein expression of Aj14-3-3ζ and promoted coelomocytes apoptosis with 5.04-fold increase in vivo, which was consistent with those from siRNA-mediated Aj14-3-3ζ knockdown assay. In contrast, miR-92a antagomir significantly elevated the mRNA and protein expression of Aj14-3-3ζ and decreased coelomocytes apoptosis. Taken together, our result confirmed that miR-92a is involved in apoptotic signaling pathway regulation perhaps via targeting Aj14-3-3ζ in sea cucumbers, which will enhance our understanding of miR-92a regulatory roles in sea cucumber pathogenesis.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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