Follicle Viability after Vitrification of Bovine Ovarian Tissue.

Autor:	Guedes JS; Clínica Pró-Criar - Reproductive Medicine, Belo Horizonte, MG, Brasil.; Post-graduation, Faculdade de Ciências Médicas de Minas Gerais, Belo Horizonte, MG, Brasil., Rodrigues JK; Rede Brasileira de Oncofertilidade - Brazilian Oncofertility Consortium, Belo Horizonte, MG, Brazil.; In Vitro Consultoria, Belo Horizonte, MG, Brazil., Campos ALM; Clínica Pró-Criar - Reproductive Medicine, Belo Horizonte, MG, Brasil.; Post-graduation, Faculdade de Ciências Médicas de Minas Gerais, Belo Horizonte, MG, Brasil., Moraes CC; Clínica Pró-Criar - Reproductive Medicine, Belo Horizonte, MG, Brasil.; Post-graduation, Faculdade de Ciências Médicas de Minas Gerais, Belo Horizonte, MG, Brasil., Caetano JPJ; Clínica Pró-Criar - Reproductive Medicine, Belo Horizonte, MG, Brasil.; Post-graduation, Faculdade de Ciências Médicas de Minas Gerais, Belo Horizonte, MG, Brasil.; Rede Brasileira de Oncofertilidade - Brazilian Oncofertility Consortium, Belo Horizonte, MG, Brazil., Marinho RM; Clínica Pró-Criar - Reproductive Medicine, Belo Horizonte, MG, Brasil.; Post-graduation, Faculdade de Ciências Médicas de Minas Gerais, Belo Horizonte, MG, Brasil.; Rede Brasileira de Oncofertilidade - Brazilian Oncofertility Consortium, Belo Horizonte, MG, Brazil.
Jazyk:	angličtina
Zdroj:	Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia [Rev Bras Ginecol Obstet] 2017 Nov; Vol. 39 (11), pp. 614-621. Date of Electronic Publication: 2017 Aug 31.
DOI:	10.1055/s-0037-1606129
Abstrakt:	Purpose  The present study aimed to evaluate the impact of vitrification on the viability of follicles using a three-dimensional (3D) in vitro culture. Methods  Bovine ovarian tissue samples ( n  = 5) obtained from slaughterhouses were utilized. The cortex was cut into small fragments of 2 × 3 × 0.5 mm using a tissue slicer. From these fragments, secondary follicles were first isolated by mechanical and enzymatic methods, then encapsulated in alginate gel and individually cultured for 20 days. Additional fragments of the same ovarian tissue were vitrified in a solution containing 25% glycerol and 25% ethylene glycol. After warming, the follicles underwent the same follicular isolation process that was performed for the fresh follicles. Results  A total of 61 follicles were isolated, 51 from fresh ovarian tissue, and 10 from vitrified tissue. After the culture, the vitrified and fresh follicles showed 20% and 43.1% survival rates respectively ( p  = 0.290), with no significant differences. At the end of the culture, there were no significant differences in follicular diameter between the vitrified (422.93 ± 85.05 µm) and fresh (412.99 ± 102.55 µm) groups ( p  = 0.725). Fresh follicles showed higher mean rate of antrum formation when compared with vitrified follicles (47.1% and 20.0% respectively), but without significant difference ( p  = 0.167). Conclusions  The follicles were able to develop, grow and form antrum in the 3D system after vitrification, despite the lower results obtained with the fresh tissue. Competing Interests: Conflicts of Interest: Authors have no conflicts of interest to disclose. (Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil.)
Databáze:	MEDLINE
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