Peculiar citric acid cycle of hydrothermal vent chemolithoautotroph Hydrogenovibrio crunogenus, and insights into carbon metabolism by obligate autotrophs.

Autor:	Quasem I; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Achille AN; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Caddick BA; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Carter TA; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Daniels C; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Delaney JA; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Delic V; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Denton KA; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Duran MC; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Fatica MK; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Ference CM; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Galkiewicz JP; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Garcia AM; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Hendrick JD; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Horton SA; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Kun MS; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Koch PW; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Lee TM; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., McCabe CR; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., McHale S; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., McDaniel LD; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Menning DM; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Menning KJ; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Mirzaei-Souderjani H; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Mostajabian S; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Nicholson DA; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Nugent CK; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Osman NP; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Pappas DI; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Rocha AM; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Rosario K; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Rubelmann H; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Schwartz JA; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Seeley KW; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Staley CM; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Wallace EM; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Wong TM; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Zielinski BL; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA., Hanson TE; School of Marine Science and Policy, Delaware Biotechnology Institute, and Department of Biological Sciences, University of Delaware, Newark, DE 19711, USA., Scott KM; Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA.
Jazyk:	angličtina
Zdroj:	FEMS microbiology letters [FEMS Microbiol Lett] 2017 Aug 01; Vol. 364 (14).
DOI:	10.1093/femsle/fnx148
Abstrakt:	The genome sequence of the obligate chemolithoautotroph Hydrogenovibrio crunogenus paradoxically predicts a complete oxidative citric acid cycle (CAC). This prediction was tested by multiple approaches including whole cell carbon assimilation to verify obligate autotrophy, phylogenetic analysis of CAC enzyme sequences and enzyme assays. Hydrogenovibrio crunogenus did not assimilate any of the organic compounds provided (acetate, succinate, glucose, yeast extract, tryptone). Enzyme activities confirmed that its CAC is mostly uncoupled from the NADH pool. 2-Oxoglutarate:ferredoxin oxidoreductase activity is absent, though pyruvate:ferredoxin oxidoreductase is present, indicating that sequence-based predictions of substrate for this oxidoreductase were incorrect, and that H. crunogenus may have an incomplete CAC. Though the H. crunogenus CAC genes encode uncommon enzymes, the taxonomic distribution of their top matches suggests that they were not horizontally acquired. Comparison of H. crunogenus CAC genes to those present in other 'Proteobacteria' reveals that H. crunogenus and other obligate autotrophs lack the functional redundancy for the steps of the CAC typical for facultative autotrophs and heterotrophs, providing another possible mechanism for obligate autotrophy. (© FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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