The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus.

Autor: Kasianov NS; Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia.; Department of Natural science, Novosibirsk National Research State University, Novosibirsk, Russia., Belousova IA; Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia.; Institute of Biology, Irkutsk State University, Irkutsk, Russia., Pavlushin SV; Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia., Dubovskiy IM; Laboratory of Insect Pathology, Institute of Systematics and Ecology of Animal SB RAS, Novosibirsk, Russia.; Novosibirsk State Agrarian University, Novosibirsk, Russia., Podgwaite JD; Northern Research Station, USDA Forest Service, Hamden, CT, United States of America., Martemyanov VV; Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia.; Biological Institute, National Research Tomsk State University, Tomsk, Russia., Bakhvalov SA; Laboratory of Insect Pathology, Institute of Systematics and Ecology of Animal SB RAS, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 Aug 30; Vol. 12 (8), pp. e0183940. Date of Electronic Publication: 2017 Aug 30 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0183940
Abstrakt: Host innate immunity is one of the factors that determines the resistance of insects to their entomopathogens. In the research reported here we studied whether or not phenoloxidase (PO), a key enzyme in the melanogenesis component of humoral immunity of insects, plays a role in the protection of Lymantria dispar larvae from infection by L. dispar multiple nucleopolyhedrovirus. We studied two types of viral infection: overt and covert. The following lines of investigation were tested: i) the intravital individual estimation of baseline PO activity in haemolymph plasma followed by virus challenging; ii) the specific inhibition of PO activity in vivo by peroral treatment of infected larvae with phenylthiourea (PTU), a competitive inhibitor of PO; iii) the evaluation of PO activity in the haemolymph plasma after larval starvation. Starvation is a stress that activates the covert infection to an overt form. All of these experiments did not show a relationship between PO activity in haemolymph plasma of L. dispar larvae and larval susceptibility to baculovirus. Moreover, starvation-induced activation of covert viral infection to an overt form occurred in 70 percent of virus-carrying larvae against the background of a dramatic increase of PO activity in haemolymph plasma in the insects studied. Our conclusion is that in L. dispar larvae PO activity is not a predictor of host resistance to baculovirus.
Databáze: MEDLINE
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