The level of Hydrogen Peroxide in HeLa Cells in an Ozonated Medium.

Autor: Kontorshchikova KN; Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia. kontclin@mail.ru., Belova AS; Federal Research Center, Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod, Russia., Dudenkova VV; Federal Research Center, Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod, Russia., Orlova AG; Federal Research Center, Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod, Russia., Terent'ev IG; Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia., Tsybusov SN; Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia., Alyasova AV; Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2017 Aug; Vol. 163 (4), pp. 570-573. Date of Electronic Publication: 2017 Aug 29.
DOI: 10.1007/s10517-017-3851-6
Abstrakt: We studied the role of hydrogen peroxide in the response of tumor cells to treatment with ozonated culture medium. Changes of the level of hydrogen peroxide in tumor cells incubated in ozonated medium were detected by using fluorescence microscopy and genetically-encoded sensor HyPer2. Modifications of fluorescent properties of the sensor reflecting accumulation of hydrogen peroxide in the cell cytoplasm were detected within 70 min from the start of exposure. The concentration of hydrogen peroxide continued to increase until 375 min. The revealed changes support the involvement of hydrogen peroxide in the cell response to ozone treatment.
Databáze: MEDLINE
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