Enterovirus D68 in Viet Nam (2009-2015).

Autor: Ny NTH; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Ho Chi Minh City University of Science, Ho Chi Minh City, Vietnam., Anh NT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Hang VTT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Nguyet LA; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Thanh TT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Ha DQ; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Minh NNQ; Children's Hospital 1, Ho Chi Minh City, Vietnam., Ha DLA; Murdoch Children's Research Institute, Melbourne, Australia., McBride A; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Tuan HM; Children's Hospital 2, Ho Chi Minh City, Vietnam., Baker S; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Tam PTT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Phuc TM; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Huong DT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Loi TQ; Dong Thap General Hospital, Ban Me Thuot City, Vietnam., Vu NTA; Dong Thap General Hospital, Ban Me Thuot City, Vietnam., Hung NV; Dak Lak General Hospital, Ban Me Thuot City, Vietnam., Minh TTT; Dak Lak General Hospital, Ban Me Thuot City, Vietnam., Xang NV; Khanh Hoa General Hospital, Nha Trang City, Vietnam., Dong N; Khanh Hoa General Hospital, Nha Trang City, Vietnam., Nghia HDT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Pham Ngoc Thach University, Ho Chi Minh City, Vietnam., Chau NVV; Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam., Thwaites G; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK., van Doorn HR; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Anscombe C; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Le Van T; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
Jazyk: angličtina
Zdroj: Wellcome open research [Wellcome Open Res] 2017 Jun 15; Vol. 2, pp. 41. Date of Electronic Publication: 2017 Jun 15 (Print Publication: 2017).
DOI: 10.12688/wellcomeopenres.11558.2
Abstrakt: Background: Since 1962, enterovirus D68 (EV-D68) has been implicated in multiple outbreaks and sporadic cases of respiratory infection worldwide, but especially in the USA and Europe with an increasing frequency between 2010 and 2014. We describe the detection, associated clinical features and molecular characterization of EV-D68 in central and southern Viet Nam between 2009 and 2015.
Methods: Enterovirus/rhinovirus PCR positive respiratory or CSF samples taken from children and adults with respiratory/central nervous system infections in Viet Nam were tested by an EV-D68 specific PCR. The included samples were derived from 3 different observational studies conducted at referral hospitals across central and southern Viet Nam between 2009 and 2015. Whole-genome sequencing was carried out using a MiSeq based approach. Phylogenetic reconstruction and estimation of evolutionary rate and recombination were carried out in BEAST and Recombination Detection Program, respectively.
Results: EV-D68 was detected in 21/625 (3.4%) enterovirus/rhinovirus PCR positive respiratory samples but in none of the 15 CSF. All the EV-D68 patients were young children (age range: 11.8 - 24.5 months) and had moderate respiratory infections. Phylogenetic analysis suggested that the Vietnamese sequences clustered with those from Asian countries, of which 9 fell in the B1 clade, and the remaining sequence was identified within the A2 clade. One intra sub-clade recombination event was detected, representing the second reported recombination within EV-D68. The evolutionary rate of EV-D68 was estimated to be 5.12E -3 substitutions/site/year. Phylogenetic analysis indicated that the virus was imported into Viet Nam in 2008.
Conclusions: We have demonstrated for the first time EV-D68 has been circulating at low levels in Viet Nam since 2008, associated with moderate acute respiratory infection in children. EV-D68 in Viet Nam is most closely related to Asian viruses, and clusters separately from recent US and European viruses that were suggested to be associated with acute flaccid paralysis.
Competing Interests: Competing interests: No competing interests were disclosed.
Databáze: MEDLINE
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