Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression.

Autor:	Deng HX; Hai-Xia Deng, Ya-Ya Yu, Ao-Qiang Zhou, Jing-Li Zhu, Li-Na Luo, Geng-Xin Chen, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong Province, China., Yu YY; Hai-Xia Deng, Ya-Ya Yu, Ao-Qiang Zhou, Jing-Li Zhu, Li-Na Luo, Geng-Xin Chen, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong Province, China., Zhou AQ; Hai-Xia Deng, Ya-Ya Yu, Ao-Qiang Zhou, Jing-Li Zhu, Li-Na Luo, Geng-Xin Chen, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong Province, China., Zhu JL; Hai-Xia Deng, Ya-Ya Yu, Ao-Qiang Zhou, Jing-Li Zhu, Li-Na Luo, Geng-Xin Chen, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong Province, China., Luo LN; Hai-Xia Deng, Ya-Ya Yu, Ao-Qiang Zhou, Jing-Li Zhu, Li-Na Luo, Geng-Xin Chen, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong Province, China., Chen WQ; Hai-Xia Deng, Ya-Ya Yu, Ao-Qiang Zhou, Jing-Li Zhu, Li-Na Luo, Geng-Xin Chen, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong Province, China., Hu L; Hai-Xia Deng, Ya-Ya Yu, Ao-Qiang Zhou, Jing-Li Zhu, Li-Na Luo, Geng-Xin Chen, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong Province, China., Chen GX; Hai-Xia Deng, Ya-Ya Yu, Ao-Qiang Zhou, Jing-Li Zhu, Li-Na Luo, Geng-Xin Chen, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong Province, China.
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2017 Aug 14; Vol. 23 (30), pp. 5538-5548.
DOI:	10.3748/wjg.v23.i30.5538
Abstrakt:	Aim: To explore the let-7a-mediated anti-cancer effect of Yangzheng Sanjie decoction (YZSJD) in gastric cancer (GC) cells. Methods: YZSJD-containing serum (YCS) was prepared using traditional Chinese medicine serum pharmacology methods. After YCS treatment, cell proliferation and apoptosis were assessed by cell counting kit-8 assay and flow cytometry, respectively, and miRNA expression profiles were determined using qPCR arrays. Let-7a expression was examined by in situ hybridization in GC tissues and by qPCR in GC cells. c-Myc protein expression was detected by immunohistochemistry in GC tissues, and by Western blot in cell lines. Results: YZSJD significantly inhibited proliferation and induced apoptosis in AGS and HS-746T GC cells. After treatment with YCS, the miRNA expression profiles were altered and the reduced let-7a levels in both cell lines were up-regulated, accompanied by a decrease in c-Myc expression. Moreover, decreased let-7a expression and increased c-Myc expression were observed during the progression of gastric mucosa cancerization. Conclusion: YZSJD inhibits proliferation and induces apoptosis of GC cells by restoring the aberrant expression of let-7a and c-Myc. Competing Interests: Conflict-of-interest statement: The authors declare that there are no conflicts of interest related to this study.
Databáze:	MEDLINE
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