Candida albicans and Pseudomonas aeruginosa Interact To Enhance Virulence of Mucosal Infection in Transparent Zebrafish.

Autor:	Bergeron AC; Department of Molecular & Biomedical Sciences, University of Maine, Orono, Maine, USA., Seman BG; Department of Molecular & Biomedical Sciences, University of Maine, Orono, Maine, USA., Hammond JH; Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA., Archambault LS; Department of Molecular & Biomedical Sciences, University of Maine, Orono, Maine, USA., Hogan DA; Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA., Wheeler RT; Department of Molecular & Biomedical Sciences, University of Maine, Orono, Maine, USA robert.wheeler1@maine.edu.; Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, Maine, USA.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2017 Oct 18; Vol. 85 (11). Date of Electronic Publication: 2017 Oct 18 (Print Publication: 2017).
DOI:	10.1128/IAI.00475-17
Abstrakt:	Polymicrobial infections often include both fungi and bacteria and can complicate patient treatment and resolution of infection. Cross-kingdom interactions among bacteria, fungi, and/or the immune system during infection can enhance or block virulence mechanisms and influence disease progression. The fungus Candida albicans and the bacterium Pseudomonas aeruginosa are coisolated in the context of polymicrobial infection at a variety of sites throughout the body, including mucosal tissues such as the lung. In vitro , C. albicans and P. aeruginosa have a bidirectional and largely antagonistic relationship. Their interactions in vivo remain poorly understood, specifically regarding host responses in mediating infection. In this study, we examine trikingdom interactions using a transparent juvenile zebrafish to model mucosal lung infection and show that C. albicans and P. aeruginosa are synergistically virulent. We find that high C. albicans burden, fungal epithelial invasion, swimbladder edema, and epithelial extrusion events serve as predictive factors for mortality in our infection model. Longitudinal analyses of fungal, bacterial, and immune dynamics during coinfection suggest that enhanced morbidity is associated with exacerbated C. albicans pathogenesis and elevated inflammation. The P. aeruginosa quorum-sensing-deficient Δ lasR mutant also enhances C. albicans pathogenicity in coinfection and induces extrusion of the swimbladder. Together, these observations suggest that C. albicans-P. aeruginosa cross talk in vivo can benefit both organisms to the detriment of the host. (Copyright © 2017 American Society for Microbiology.)
Databáze:	MEDLINE
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