Tannic acid affects the phenotype of Staphylococcus aureus resistant to tetracycline and erythromycin by inhibition of efflux pumps.

Autor: Tintino SR; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry/CCBS/URCA, Brazil., Morais-Tintino CD; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry/CCBS/URCA, Brazil., Campina FF; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry/CCBS/URCA, Brazil., Costa MDS; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry/CCBS/URCA, Brazil., Menezes IRA; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry/CCBS/URCA, Brazil., de Matos YMLS; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry/CCBS/URCA, Brazil., Calixto-Júnior JT; Juazeiro do Norte College (FJN), Brazil., Pereira PS; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry/CCBS/URCA, Brazil., Siqueira-Junior JP; Laboratory of Microrganism Genetics (LGM), Department of Molecular Biology/CCEN/UFPB, Brazil; Aggeu Magalhães Research Center, CPqAM/Fiocruz, Department of Microbiology, Brazil., Leal-Balbino TC; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry/CCBS/URCA, Brazil., Coutinho HDM; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry/CCBS/URCA, Brazil. Electronic address: hdmcoutinho@gmail.com., Balbino VQ; Evolutionary Biology and Bioinformatics Laboratory (LABBE), Department of Genetics|CCB|UFPE, Brazil.
Jazyk: angličtina
Zdroj: Bioorganic chemistry [Bioorg Chem] 2017 Oct; Vol. 74, pp. 197-200. Date of Electronic Publication: 2017 Aug 12.
DOI: 10.1016/j.bioorg.2017.08.004
Abstrakt: The widespread use of antibiotics created selective pressure for the emergence of strains that would persist despite antibiotic toxicity. The bacterial resistance mechanisms are several, with efflux pumps being one of the main ones. These pumps are membrane proteins with the function of removing antibiotics from the cell cytoplasm. Due to this importance, the aim of this work was to evaluate the inhibitory effect of tannic acid against efflux pumps expressed by the Staphylococcus aureus RN4220 and IS-58 strains. The efflux pump inhibition was assayed using a sub-inhibitory concentration of efflux pump standard inhibitors and tannic acid (MIC/8), observing their capacity to decrease the MIC of Ethidium bromide (EtBr) and antibiotics due the possible inhibitory effect of these substances. The MICs of EtBr and antibiotics were significantly different in the presence of tannic acid, indicating the inhibitory effect of this product against efflux pumps of both strains. These results indicate the possible usage of tannic acid asan inhibitor and an adjuvant in the antibiotic therapy against multidrug resistant bacteria (MDR).
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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