cDNA microarray analysis of human keratinocytes cells of patients submitted to chemoradiotherapy and oral photobiomodulation therapy: pilot study.

Autor: Antunes HS; Clinical Research Division, Instituto Nacional de Câncer (INCA), Rua André Cavalcante, n 37, 2 andar, Rio de Janeiro, RJ, 20231-050, Brazil. hspindola@inca.gov.br., Wajnberg G; Bioinformatics Unit, Clinical Research Coordination, Instituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, Brazil.; Head and Neck Oncology Group, Grupo de Oncologia D'Or, Rio de Janeiro, Brazil.; Radiation Oncology Division, INCA, Rio de Janeiro, Brazil.; Head and Neck Surgery Division, INCA, Rio de Janeiro, Brazil.; Program of Cellular Biology, INCA, Rio de Janeiro, Brazil.; Department of Immunobiology, Universidade Federal Fluminense (UFF), Niterói, Brazil.; Laboratory of Functional Genomics and Bioinformatics, Oswaldo Cruz Institute, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil., Pinho MB; Clinical Research Division, Instituto Nacional de Câncer (INCA), Rua André Cavalcante, n 37, 2 andar, Rio de Janeiro, RJ, 20231-050, Brazil., Jorge NAN; Bioinformatics Unit, Clinical Research Coordination, Instituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, Brazil.; Head and Neck Oncology Group, Grupo de Oncologia D'Or, Rio de Janeiro, Brazil.; Radiation Oncology Division, INCA, Rio de Janeiro, Brazil.; Head and Neck Surgery Division, INCA, Rio de Janeiro, Brazil.; Program of Cellular Biology, INCA, Rio de Janeiro, Brazil.; Department of Immunobiology, Universidade Federal Fluminense (UFF), Niterói, Brazil.; Laboratory of Functional Genomics and Bioinformatics, Oswaldo Cruz Institute, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil., de Moraes JLM; Clinical Research Division, Instituto Nacional de Câncer (INCA), Rua André Cavalcante, n 37, 2 andar, Rio de Janeiro, RJ, 20231-050, Brazil., Stefanoff CG; Clinical Research Division, Instituto Nacional de Câncer (INCA), Rua André Cavalcante, n 37, 2 andar, Rio de Janeiro, RJ, 20231-050, Brazil., Herchenhorn D; Head and Neck Oncology Group, Grupo de Oncologia D'Or, Rio de Janeiro, Brazil., Araújo CMM; Radiation Oncology Division, INCA, Rio de Janeiro, Brazil., Viégas CMP; Radiation Oncology Division, INCA, Rio de Janeiro, Brazil., Rampini MP; Clinical Research Division, Instituto Nacional de Câncer (INCA), Rua André Cavalcante, n 37, 2 andar, Rio de Janeiro, RJ, 20231-050, Brazil., Dias FL; Head and Neck Surgery Division, INCA, Rio de Janeiro, Brazil., de Araujo-Souza PS; Program of Cellular Biology, INCA, Rio de Janeiro, Brazil.; Department of Immunobiology, Universidade Federal Fluminense (UFF), Niterói, Brazil., Passetti F; Bioinformatics Unit, Clinical Research Coordination, Instituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, Brazil.; Head and Neck Oncology Group, Grupo de Oncologia D'Or, Rio de Janeiro, Brazil.; Radiation Oncology Division, INCA, Rio de Janeiro, Brazil.; Head and Neck Surgery Division, INCA, Rio de Janeiro, Brazil.; Program of Cellular Biology, INCA, Rio de Janeiro, Brazil.; Department of Immunobiology, Universidade Federal Fluminense (UFF), Niterói, Brazil.; Laboratory of Functional Genomics and Bioinformatics, Oswaldo Cruz Institute, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil., Ferreira CG; D'Or Institute for Research and Education, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2018 Jan; Vol. 33 (1), pp. 11-18. Date of Electronic Publication: 2017 Aug 24.
DOI: 10.1007/s10103-017-2313-8
Abstrakt: Oral mucositis is an acute toxicity that occurs in patients submitted to chemoradiotherapy to treat head and neck squamous cell carcinoma. In this study, we evaluated differences in gene expression in the keratinocytes of the oral mucosa of patients treated with photobiomodulation therapy and tried to associate the molecular mechanisms with clinical findings. From June 2009 to December 2010, 27 patients were included in a randomized double-blind pilot study. Buccal smears from 13 patients were obtained at days 1 and 10 of chemoradiotherapy, and overall gene expression of samples from both dates were analyzed by complementary DNA (cDNA) microarray. In addition, samples from other 14 patients were also collected at D1 and D10 of chemoradiotherapy for subsequent validation of cDNA microarray findings by qPCR. The expression array analysis identified 105 upregulated and 60 downregulated genes in our post-treatment samples when compared with controls. Among the upregulated genes with the highest fold change, it was interesting to observe the presence of genes related to keratinocyte differentiation. Among downregulated genes were observed genes related to cytotoxicity and immune response. The results indicate that genes known to be induced during differentiation of human epidermal keratinocytes were upregulated while genes associated with cytotoxicity and immune response were downregulated in the laser group. These results support previous clinical findings indicating that the lower incidence of oral mucositis associated with photobiomodulation therapy might be correlated to the activation of genes involved in keratinocyte differentiation.
Databáze: MEDLINE
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