Development of novel monoclonal antibodies against starch and ulvan - implications for antibody production against polysaccharides with limited immunogenicity.

Autor: Rydahl MG; Department of Plant and Environmental Sciences, DK-1871, Frederiksberg, Denmark. mgr@plen.ku.dk., Krac Un SK; Department of Plant and Environmental Sciences, DK-1871, Frederiksberg, Denmark., Fangel JU; Carlsberg Research Laboratory, J.C. Jacobsens Gade 4, DK-1799, Copenhagen V, Denmark., Michel G; Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, Roscoff, Bretagne, France., Guillouzo A; Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, Roscoff, Bretagne, France., Génicot S; Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, Roscoff, Bretagne, France., Mravec J; Department of Plant and Environmental Sciences, DK-1871, Frederiksberg, Denmark., Harholt J; Carlsberg Research Laboratory, J.C. Jacobsens Gade 4, DK-1799, Copenhagen V, Denmark., Wilkens C; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800, Lyngby, Denmark., Motawia MS; Department of Plant and Environmental Sciences, DK-1871, Frederiksberg, Denmark., Svensson B; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800, Lyngby, Denmark., Tranquet O; UR1268 Biopolymeres, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, F-44316, Nantes, France., Ralet MC; UR1268 Biopolymeres, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, F-44316, Nantes, France., Jørgensen B; Department of Plant and Environmental Sciences, DK-1871, Frederiksberg, Denmark., Domozych DS; Biology Department, Skidmore College, Saratoga Springs, NY, 12866, USA., Willats WGT; School of Agriculture, Food and Rural Development, Newcastle University, NE1 7RU, Newcastle upon Tyne, UK. william.willats@newcastle.ac.uk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Aug 24; Vol. 7 (1), pp. 9326. Date of Electronic Publication: 2017 Aug 24.
DOI: 10.1038/s41598-017-04307-2
Abstrakt: Monoclonal antibodies (mAbs) are widely used and powerful research tools, but the generation of mAbs against glycan epitopes is generally more problematic than against proteins. This is especially significant for research on polysaccharide-rich land plants and algae (Viridiplantae). Most antibody production is based on using single antigens, however, there are significant gaps in the current repertoire of mAbs against some glycan targets with low immunogenicity. We approached mAb production in a different way and immunised with a complex mixture of polysaccharides. The multiplexed screening capability of carbohydrate microarrays was then exploited to deconvolute the specificities of individual mAbs. Using this strategy, we generated a set of novel mAbs, including one against starch (INCh1) and one against ulvan (INCh2). These polysaccharides are important storage and structural polymers respectively, but both are generally considered as having limited immunogenicity. INCh1 and INCh2 therefore represent important new molecular probes for Viridiplantae research. Moreover, since the α-(1-4)-glucan epitope recognised by INCh1 is also a component of glycogen, this mAb can also be used in mammalian systems. We describe the detailed characterisation of INCh1 and INCh2, and discuss the potential of a non-directed mass-screening approach for mAb production against some glycan targets.
Databáze: MEDLINE
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