Composition and Control of a Deg/ENaC Channel during Presynaptic Homeostatic Plasticity.

Autor:	Orr BO; Department of Biochemistry and Biophysics, Kavli Institute for Fundamental Neuroscience, University of California, San Francisco, San Francisco, CA 94158, USA., Gorczyca D; Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, USA., Younger MA; Department of Biochemistry and Biophysics, Kavli Institute for Fundamental Neuroscience, University of California, San Francisco, San Francisco, CA 94158, USA., Jan LY; Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, USA., Jan YN; Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, USA., Davis GW; Department of Biochemistry and Biophysics, Kavli Institute for Fundamental Neuroscience, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: graeme.davis@ucsf.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2017 Aug 22; Vol. 20 (8), pp. 1855-1866.
DOI:	10.1016/j.celrep.2017.07.074
Abstrakt:	The homeostatic control of presynaptic neurotransmitter release stabilizes information transfer at synaptic connections in the nervous system of organisms ranging from insect to human. Presynaptic homeostatic signaling centers upon the regulated membrane insertion of an amiloride-sensitive degenerin/epithelial sodium (Deg/ENaC) channel. Elucidating the subunit composition of this channel is an essential step toward defining the underlying mechanisms of presynaptic homeostatic plasticity (PHP). Here, we demonstrate that the ppk1 gene encodes an essential subunit of this Deg/ENaC channel, functioning in motoneurons for the rapid induction and maintenance of PHP. We provide genetic and biochemical evidence that PPK1 functions together with PPK11 and PPK16 as a presynaptic, hetero-trimeric Deg/ENaC channel. Finally, we highlight tight control of Deg/ENaC channel expression and activity, showing increased PPK1 protein expression during PHP and evidence for signaling mechanisms that fine tune the level of Deg/ENaC activity during PHP. (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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