The impact of ageing, fasting and high-fat diet on central and peripheral glucose tolerance and glucose-sensing neural networks in the arcuate nucleus.

Autor: van den Top M; NeuroSolutions Ltd, Coventry, UK., Zhao FY; NeuroSolutions Ltd, Coventry, UK., Viriyapong R; Warwick Medical School, University of Warwick, Coventry, UK.; MOAC DTC, University of Warwick, Coventry, UK., Michael NJ; Metabolic Disease and Obesity Program, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia.; Department of Physiology, Monash University, Clayton, VIC, Australia., Munder AC; Metabolic Disease and Obesity Program, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia.; Department of Physiology, Monash University, Clayton, VIC, Australia., Pryor JT; Warwick Medical School, University of Warwick, Coventry, UK.; Metabolic Disease and Obesity Program, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia.; Department of Physiology, Monash University, Clayton, VIC, Australia., Renaud LP; Ottawa Hospital Research Institute, Ottawa Civic Hospital, Ottawa, ON, Canada., Spanswick D; NeuroSolutions Ltd, Coventry, UK.; Warwick Medical School, University of Warwick, Coventry, UK.; Metabolic Disease and Obesity Program, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia.; Department of Physiology, Monash University, Clayton, VIC, Australia.; Neuroscience Program, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia.
Jazyk: angličtina
Zdroj: Journal of neuroendocrinology [J Neuroendocrinol] 2017 Oct; Vol. 29 (10).
DOI: 10.1111/jne.12528
Abstrakt: Obesity and ageing are risk factors for diabetes. In the present study, we investigated the effects of ageing, obesity and fasting on central and peripheral glucose tolerance and on glucose-sensing neuronal function in the arcuate nucleus of rats, with a view to providing insight into the central mechanisms regulating glucose homeostasis and how they change or are subject to dysfunction with ageing and obesity. We show that, following a glucose load, central glucose tolerance at the level of the cerebrospinal fluid (CSF) and plasma is significantly reduced in rats maintained on a high-fat diet (HFD). With ageing, up to 2 years, central glucose tolerance was impaired in an age-dependent manner, whereas peripheral glucose tolerance remained unaffected. Ageing-induced peripheral glucose intolerance was improved by a 24-hour fast, whereas central glucose tolerance was not corrected. Pre-wean, immature animals have elevated basal plasma glucose levels and a delayed increase in central glucose levels following peripheral glucose injection compared to mature animals. Electrophysiological recording techniques revealed an energy-status-dependent role for glucose-excited, inhibited and adapting neurones, along with glucose-induced changes in synaptic transmission. We conclude that ageing affects central glucose tolerance, whereas HFD profoundly affects central and peripheral glucose tolerance and, in addition, glucose-sensing neurones adapt function in an energy-status-dependent manner.
(© 2017 British Society for Neuroendocrinology.)
Databáze: MEDLINE
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