RNA-editing enzymes ADAR1 and ADAR2 coordinately regulate the editing and expression of Ctn RNA.

Autor: Anantharaman A; Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, IL, USA., Gholamalamdari O; Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, IL, USA., Khan A; Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, IL, USA., Yoon JH; Laboratory of Genetics and Genomics, National Institute of Aging-Intramural Research Program, NIH, Baltimore, MD, USA., Jantsch MF; Department for Medical Biochemistry, Center for Anatomy and Cell Biology, Medical University of Vienna, Austria., Hartner JC; Horizon Discovery, Cambridge, UK., Gorospe M; Laboratory of Genetics and Genomics, National Institute of Aging-Intramural Research Program, NIH, Baltimore, MD, USA., Prasanth SG; Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, IL, USA., Prasanth KV; Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, IL, USA.
Jazyk: angličtina
Zdroj: FEBS letters [FEBS Lett] 2017 Sep; Vol. 591 (18), pp. 2890-2904. Date of Electronic Publication: 2017 Aug 30.
DOI: 10.1002/1873-3468.12795
Abstrakt: Adenosine deaminases acting on RNA (ADARs) are proteins that catalyse widespread A-to-I editing within RNA sequences. We recently reported that ADAR2 edits and stabilizes nuclear-retained Cat2 transcribed nuclear RNA (Ctn RNA). Here, we report that ADAR1 coordinates with ADAR2 to regulate editing and stability of Ctn RNA. We observe an RNA-dependent interaction between ADAR1 and ADAR2. Furthermore, ADAR1 negatively regulates interaction of Ctn RNA with RNA-destabilizing proteins. We also show that breast cancer (BC) cells display elevated ADAR1 but not ADAR2 levels, compared to nontumourigenic cells. Additionally, BC patients with elevated levels of ADAR1 show low survival. Our findings provide insights into overlapping substrate preferences of ADARs and potential involvement of ADAR1 in BC.
(© 2017 Federation of European Biochemical Societies.)
Databáze: MEDLINE
Externí odkaz: