IL-18 associated with lung lymphoid aggregates drives IFNγ production in severe COPD.

Autor:	Briend E; MedImmune Ltd, Granta Park, Cambridge, CB21 6GH, UK.; Present address: Agenus Ltd, Cambridge, UK., Ferguson GJ; MedImmune Ltd, Granta Park, Cambridge, CB21 6GH, UK., Mori M; Department of Experimental Medical Science, BMC D12, Lund University, SE-221 84, Lund, Sweden., Damera G; MedImmune LLC, 1 MedImmune Way, Gaithersburg, MD, USA., Stephenson K; MedImmune Ltd, Granta Park, Cambridge, CB21 6GH, UK.; Present address: University of Nottingham, Nottingham, UK., Karp NA; Quantitative Biology IMED, AstraZeneca R&D, Cambridge, UK., Sethi S; Department of Medicine, University at Buffalo, 3495 Bailey Avenue, Buffalo, NY, 14215, USA., Ward CK; MedImmune LLC, 1 MedImmune Way, Gaithersburg, MD, USA.; Present address: Bristol-Myers Squibb, Princeton, NJ, USA., Sleeman MA; MedImmune Ltd, Granta Park, Cambridge, CB21 6GH, UK.; Present address: Regeneron Pharmaceuticals Inc, Tarrytown, NY, USA., Erjefält JS; Department of Experimental Medical Science, BMC D12, Lund University, SE-221 84, Lund, Sweden.; Department of Respiratory Medicine and Allergology, Lund University Hospital, Lund, Sweden., Finch DK; MedImmune Ltd, Granta Park, Cambridge, CB21 6GH, UK. Finchd@medimmune.com.
Jazyk:	angličtina
Zdroj:	Respiratory research [Respir Res] 2017 Aug 22; Vol. 18 (1), pp. 159. Date of Electronic Publication: 2017 Aug 22.
DOI:	10.1186/s12931-017-0641-7
Abstrakt:	Background: Increased interferon gamma (IFNγ) release occurs in Chronic Obstructive Pulmonary Disease (COPD) lungs. IFNγ supports optimal viral clearance, but if dysregulated could increase lung tissue destruction. Methods: The present study investigates which mediators most closely correlate with IFNγ in sputum in stable and exacerbating disease, and seeks to shed light on the spatial requirements for innate production of IFNγ, as reported in mouse lymph nodes, to observe whether such microenvironmental cellular organisation is relevant to IFNγ production in COPD lung. Results: We show tertiary follicle formation in severe disease alters the dominant mechanistic drivers of IFNγ production, because cells producing interleukin-18, a key regulator of IFNγ, are highly associated with such structures. Interleukin-1 family cytokines correlated with IFNγ in COPD sputum. We observed that the primary source of IL-18 in COPD lungs was myeloid cells within lymphoid aggregates and IL-18 was increased in severe disease. IL-18 released from infected epithelium or from activated myeloid cells, was more dominant in driving IFNγ when IL-18-producing and responder cells were in close proximity. Conclusions: Unlike tight regulation to control infection spread in lymphoid organs, this local interface between IL-18-expressing and responder cell is increasingly supported in lung as disease progresses, increasing its potential to increase tissue damage via IFNγ.
Databáze:	MEDLINE
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