Analysis of Mendelian inheritance and genetic linkage in microsatellite loci of Eucalyptus urophylla S.T. Blake.

Autor: Pupin S; Departamento de Fitotecnia, Tecnologia de Alimentos e Sócio Economia, Faculdade de Engenharia, Universidade Estadual Paulista, Ilha Solteira, SP, Brasil silvelise.pupin@gmail.com., Rosse LN; Eldorado Brasil, Três Lagoas, MS, Brasil., Souza ICG; Suzano Papel e Celulose, Itapetininga, SP, Brasil., Cambuim J; Departamento de Fitotecnia, Tecnologia de Alimentos e Sócio Economia, Faculdade de Engenharia, Universidade Estadual Paulista, Ilha Solteira, SP, Brasil., Marino CL; Departamento de Genética, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brasil., Moraes MLT; Departamento de Fitotecnia, Tecnologia de Alimentos e Sócio Economia, Faculdade de Engenharia, Universidade Estadual Paulista, Ilha Solteira, SP, Brasil., Sebbenn AM; , Brasil.
Jazyk: angličtina
Zdroj: Genetics and molecular research : GMR [Genet Mol Res] 2017 Aug 17; Vol. 16 (3). Date of Electronic Publication: 2017 Aug 17.
DOI: 10.4238/gmr16039713
Abstrakt: Eucalyptus urophylla is an important species in the Brazilian forest sector due to its rapid growth rates and resistance to disease. The aim of this study was to verify Mendelian inheritance, genetic linkage, and genotypic disequilibrium for 15 microsatellite loci, with the goal of producing a robust set of genetic markers. Mendelian inheritance and genetic linkage analyses were carried out using genotypes from maternal trees, and their open-pollinated seeds and genotypic disequilibrium were assessed using adult trees. By comparing heterozygous maternal genotypes and their seeds, we found no significant deviations from the expected 1:1 Mendelian segregation and the expected 1:1:1:1 segregation hypothesis for pairwise loci. For adult trees, we did not find strong evidence of genotypic imbalance for pairwise loci. Our results indicated that the analyzed set of microsatellite loci could be used to carry out analyses of genetic diversity, mating system, and parentage in E. urophylla.
Databáze: MEDLINE