Architecture and Dynamics of the Jasmonic Acid Gene Regulatory Network.

Autor:	Hickman R; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Van Verk MC; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands.; Bioinformatics, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Van Dijken AJH; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Mendes MP; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Vroegop-Vos IA; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Caarls L; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Steenbergen M; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Van der Nagel I; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Wesselink GJ; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Jironkin A; Warwick Systems Biology Centre, University of Warwick, Coventry CV4 7AL, United Kingdom., Talbot A; School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.; Department of Biology, University of York, York YO10 5DD, United Kingdom., Rhodes J; Warwick Systems Biology Centre, University of Warwick, Coventry CV4 7AL, United Kingdom., De Vries M; Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Schuurink RC; Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Denby K; Warwick Systems Biology Centre, University of Warwick, Coventry CV4 7AL, United Kingdom.; School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.; Department of Biology, University of York, York YO10 5DD, United Kingdom., Pieterse CMJ; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands., Van Wees SCM; Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB, Utrecht, The Netherlands s.vanwees@uu.nl.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2017 Sep; Vol. 29 (9), pp. 2086-2105. Date of Electronic Publication: 2017 Aug 21.
DOI:	10.1105/tpc.16.00958
Abstrakt:	Jasmonic acid (JA) is a critical hormonal regulator of plant growth and defense. To advance our understanding of the architecture and dynamic regulation of the JA gene regulatory network, we performed a high-resolution RNA-seq time series of methyl JA-treated Arabidopsis thaliana at 15 time points over a 16-h period. Computational analysis showed that methyl JA (MeJA) induces a burst of transcriptional activity, generating diverse expression patterns over time that partition into distinct sectors of the JA response targeting specific biological processes. The presence of transcription factor (TF) DNA binding motifs correlated with specific TF activity during temporal MeJA-induced transcriptional reprogramming. Insight into the underlying dynamic transcriptional regulation mechanisms was captured in a chronological model of the JA gene regulatory network. Several TFs, including MYB59 and bHLH27, were uncovered as early network components with a role in pathogen and insect resistance. Analysis of subnetworks surrounding the TFs ORA47, RAP2.6L, MYB59, and ANAC055, using transcriptome profiling of overexpressors and mutants, provided insights into their regulatory role in defined modules of the JA network. Collectively, our work illuminates the complexity of the JA gene regulatory network, pinpoints and validates previously unknown regulators, and provides a valuable resource for functional studies on JA signaling components in plant defense and development. (© 2017 American Society of Plant Biologists. All rights reserved.)
Databáze:	MEDLINE
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