Tempol improves lipid profile and prevents left ventricular hypertrophy in LDL receptor gene knockout (LDLr-/-) mice on a high-fat diet.

Autor:	Viana Gonçalves IC; Faculdade de Medicina, Universidade José do Rosário Vellano (UNIFENAS), Alfenas, MG, Brazil., Cerdeira CD; Departamento de Bioquímica (DBq), Instituto de Ciências Biomédicas, Universidade Federal de Alfenas (UNIFAL-MG), Alfenas, MG, Brazil. Electronic address: daniel.cerdeira.84@gmail.com., Poletti Camara E; Faculdade de Medicina, Universidade José do Rosário Vellano (UNIFENAS), Alfenas, MG, Brazil., Dias Garcia JA; Faculdade de Medicina, Universidade José do Rosário Vellano (UNIFENAS), Alfenas, MG, Brazil., Ribeiro Pereira Lima Brigagão M; Departamento de Bioquímica (DBq), Instituto de Ciências Biomédicas, Universidade Federal de Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., Bessa Veloso Silva R; Faculdade de Medicina, Universidade José do Rosário Vellano (UNIFENAS), Alfenas, MG, Brazil., Bitencourt Dos Santos G; Faculdade de Medicina, Universidade José do Rosário Vellano (UNIFENAS), Alfenas, MG, Brazil.
Jazyk:	English; Portuguese
Zdroj:	Revista portuguesa de cardiologia : orgao oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology [Rev Port Cardiol] 2017 Sep; Vol. 36 (9), pp. 629-638. Date of Electronic Publication: 2017 Aug 18.
DOI:	10.1016/j.repc.2017.02.014
Abstrakt:	Introduction and Objective: Dyslipidemia is associated with increased risk of cardiovascular disease and atherosclerosis, and hence with high morbidity and mortality. This study investigated the effects of the nitroxide 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (Tempol) on lipid profile and cardiac morphology in low-density lipoprotein (LDL) receptor gene knockout (LDLr-/-) mice. Methods: Male LDLr-/- mice (three months old, approximately 22 g weight) were divided into the following groups: controls, including (1) standard chow (SC, n=8) and (2) high-fat diet (HFD, n=8); and treatment, including (3) standard chow + Tempol (SC+T, n=8) (30 mg/kg administered by gavage, once daily) and (4) high-fat diet + Tempol (HFD+T, n=8) (30 mg/kg). After 30 days of the diet/treatment, whole blood was collected for analysis of biochemical parameters (total cholesterol, triglycerides [TG], high-density lipoprotein [HDL], LDL, and very low-density lipoprotein [VLDL]). The heart was removed through thoracotomy and histological analysis of the left ventricle was performed. Results: A significant increase in TG, LDL, and VLDL and marked left ventricular hypertrophy (LVH) were demonstrated in the HFD group relative to the SC group (p<0.05), while Tempol treatment (HFD+T group) significantly (p<0.05) prevented increases in the levels of these lipid profile markers and attenuated LVH compared with the HFD group. Conclusion: In this study, Tempol showed potential for the prevention of events related to serious diseases of the cardiovascular system. (Copyright © 2017 Sociedade Portuguesa de Cardiologia. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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