| Autor: |
Almasry SM; 1 Department of Anatomy, Almansoura University, Egypt.; 2 Department of Anatomy, Almadinah Almunawarah, Taibah University, Saudi Arabia., Elfayomy AK; 3 Department of Obstetrics and Gynecology, Zagazig University, Zagazig, Egypt.; 4 Department of Obstetrics and Gynecology, Almadinah Almunawarah, Taibah University, Saudi Arabia., El-Sherbiny MH; 2 Department of Anatomy, Almadinah Almunawarah, Taibah University, Saudi Arabia.; 5 Department of Anatomy, Amaarefa College of Medicine, Riyadh, Saudi Arabia. |
| Jazyk: |
angličtina |
| Zdroj: |
Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2018 May; Vol. 25 (5), pp. 773-781. Date of Electronic Publication: 2017 Aug 22. |
| DOI: |
10.1177/1933719117725824 |
| Abstrakt: |
In this study, we describe a novel insight about the use of bone marrow-derived mesenchymal stem cells (BM-MSCs) for fallopian tube (FT) regeneration. Seventy rats' tubes were involved in this study and divided into 4 groups: control (15), ethanol injured (20), mesenchymal stem cell (MSC)-recipient without injury (15), and MSC recipient after injury (20). The BM-MSCs were isolated from male rats, and their incorporation into the tissues was confirmed by the detection of Sry gene in MSC-recipient rats using RT-PCR. Histological and immunohistological sections of the 4 groups were comparably evaluated. We found that direct injection of ethanol into FT caused structural impairment, which was restored largely after receiving MSCs. We have revealed for the first time that prominin 1 (Prom1, stem cell marker) was expressed in the fimbriated distal tubal end. The MSC transplantation caused (1) significant increase in the tissue level and immunoexpresstion of Prom1 ( P < .001 and P = .017, respectively) and vascular endothelial growth factor (VEGF; vasculogenic marker; P < .001 and P = .004, respectively), (2) significant increase in the immunoexpresstion of proliferating cell nuclear antigen (PCNA; proliferation marker; P < .001), and (3) significant decrease in the immunoexpresstion of caspase 3 (CASP-3; apoptotic marker; P < .001) compared to the injured tissues. In conclusion, MSCs could exhibit its restorative effect on FT through their ability to (1) activate the resident stem cells in the distal tubal end, (2) mediate the expression of VEGF and PCNA, and (3) influence tissue apoptosis. This study laid the foundation for assessing the contribution of stem cells in the distal tubal end in direct repair of the tube when required to assist reproduction. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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