Cross-reactivity and inhibition myotoxic effects induced by Bothrops snake venoms using specific polyclonal anti-BnSP7 antibodies.

Autor: Melo LL; Instituto de Biologia, Universidade Federal de Uberlândia, Av. Pará, 1720, Campus Umuarama, Bloco 2D, Uberlândia, MG, 38400-902, Brazil; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará, 1720, Campus Umuarama, Bloco 2E, Uberlândia, MG, 38400-902, Brazil., Mendes MM; Instituto de Ciências Biológicas, Universidade Federal de Goiás, BR 364, km 195, nº 3800, Campus Cidade Universitária, Jataí, 75801-615, GO, Brazil., Alves LM; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará, 1720, Campus Umuarama, Bloco 2E, Uberlândia, MG, 38400-902, Brazil., Isabel TF; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará, 1720, Campus Umuarama, Bloco 2E, Uberlândia, MG, 38400-902, Brazil., Vieira SAPB; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará, 1720, Campus Umuarama, Bloco 2E, Uberlândia, MG, 38400-902, Brazil., Gimenes SNC; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará, 1720, Campus Umuarama, Bloco 2E, Uberlândia, MG, 38400-902, Brazil., Soares AM; Fundação Oswaldo Cruz, Rua da Beira, 7671, BR 364, Km 3,5, Porto Velho, RO, 76812-245 Brazil., Rodrigues VM; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará, 1720, Campus Umuarama, Bloco 2E, Uberlândia, MG, 38400-902, Brazil., Izidoro LFM; Faculdade de Medicina, Universidade Federal de Uberlândia, Av. Pará, 1720, Campus Umuarama, Bloco 2U, Uberlândia, MG, 38400-902, Brazil. Electronic address: luiz.izidoro@ufu.br.
Jazyk: angličtina
Zdroj: Biologicals : journal of the International Association of Biological Standardization [Biologicals] 2017 Nov; Vol. 50, pp. 109-116. Date of Electronic Publication: 2017 Aug 16.
DOI: 10.1016/j.biologicals.2017.08.002
Abstrakt: Polyclonal antibodies raised in Balb-c mice against BnSP-7, a Lys-49 phospholipase A2, were used to measure cross reactivity against other snake venoms. Using ELISA, these antibodies were able to recognize PLA 2 s isoforms present in venoms of botropic snakes at 1:6400, 1:3200 and 1:100 ratios (w/w). These antibodies highly recognized proteins of low molecular weight (∼14,000) from crude snake venom Bp and Bm by Western Blotting. PLA 2 these venoms, by alignment of primary structures demonstrated high identity with BnSP-7 PLA 2 , especially in the C-terminal region. However, the crude snake venom Bd and Bj, showed low recognition. The PLA 2 activity of Bothrops pauloensis, Bothrops moojeni venoms or BpPLA 2 -TXI was inhibited significantly when anti-BnSP-7 antibodies were incubated at 1:10 and 1:20 ratios (venoms or toxin:anti-BnSP-7, w/w), respectively. The myotoxic effect induced by the same venoms was also reduced significantly at 1:1, 1:10 and 1:20 ratios, by decreased creatine kinase levels. The anti-PLA2 polyclonal antibodies effectively recognized PLA2s from Bothrops pauloensis and Bothrops moojeni venoms, and neutralized specific catalytic and myotoxic activity.
(Copyright © 2017 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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