Large-scale Proteomics Combined with Transgenic Experiments Demonstrates An Important Role of Jasmonic Acid in Potassium Deficiency Response in Wheat and Rice.

Autor:	Li G; From the ‡National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China.; §Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou, 450002, China., Wu Y; From the ‡National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China., Liu G; From the ‡National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China., Xiao X; §Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou, 450002, China., Wang P; From the ‡National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China., Gao T; From the ‡National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China., Xu M; §Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou, 450002, China., Han Q; ¶National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou, 450002, China., Wang Y; From the ‡National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China.; ¶National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou, 450002, China., Guo T; From the ‡National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China.; ¶National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou, 450002, China., Kang G; From the ‡National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China; guozhangkang@henau.edu.cn.; §Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou, 450002, China.
Jazyk:	angličtina
Zdroj:	Molecular & cellular proteomics : MCP [Mol Cell Proteomics] 2017 Nov; Vol. 16 (11), pp. 1889-1905. Date of Electronic Publication: 2017 Aug 18.
DOI:	10.1074/mcp.RA117.000032
Abstrakt:	Potassium (K + ) is the most abundant inorganic cation in plants, and molecular dissection of K + deficiency has received considerable interest in order to minimize K + fertilizer input and develop high quality K + -efficient crops. However, the molecular mechanism of plant responses to K + deficiency is still poorly understood. In this study, 2-week-old bread wheat seedlings grown hydroponically in Hoagland solution were transferred to K + -free conditions for 8 d, and their root and leaf proteome profiles were assessed using the iTRAQ proteome method. Over 4000 unique proteins were identified, and 818 K + -responsive protein species showed significant differences in abundance. The differentially expressed protein species were associated with diverse functions and exhibited organ-specific differences. Most of the differentially expressed protein species related to hormone synthesis were involved in jasmonic acid (JA) synthesis and the upregulated abundance of JA synthesis-related enzymes could result in the increased JA concentrations. Abundance of allene oxide synthase (AOS), one key JA synthesis-related enzyme, was significantly increased in K + -deficient wheat seedlings, and its overexpression markedly increased concentrations of K + and JA, altered the transcription levels of some genes encoding K + -responsive protein species, as well as enhanced the tolerance of rice plants to low K + or K + deficiency. Moreover, rice AOS mutant ( osaos ) exhibited more sensitivity to low K + or K + deficiency. Our findings could highlight the importance of JA in K + deficiency, and imply a network of molecular processes underlying plant responses to K + deficiency. (© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.)
Databáze:	MEDLINE
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