| Autor: |
Zape JP; a Cardiovascular Research Institute , University of California San Francisco , San Francisco , CA , USA., Lizama CO; a Cardiovascular Research Institute , University of California San Francisco , San Francisco , CA , USA., Cautivo KM; c Department of Laboratory of Medicine , University of California San Francisco, School of Medicine , San Francisco , CA , USA., Zovein AC; a Cardiovascular Research Institute , University of California San Francisco , San Francisco , CA , USA.; b Department of Pediatrics, Division of Neonatology , University of California San Francisco School of Medicine , San Francisco , CA , USA. |
| Abstrakt: |
The emergence of haematopoietic stem and progenitor cells (HSPCs) from hemogenic endothelium results in the formation of sizeable HSPC clusters attached to the vascular wall. We evaluate the cell cycle and proliferation of HSPCs involved in cluster formation, as well as the molecular signatures from their initial appearance to the point when cluster cells are capable of adult engraftment (definitive HSCs). We uncover a non-clonal origin of HSPC clusters with differing cell cycle, migration, and cell signaling attributes. In addition, we find that the complement cascade is highly enriched in mature HSPC clusters, possibly delineating a new role for this pathway in engraftment. |
