Design and synthesis of multiple antigenic peptides and their application for dengue diagnosis.

Autor: Rai R; Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. Electronic address: reetar27@gmail.com., Dubey S; Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India., Santosh KV; Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India., Biswas A; Department of Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India., Mehrotra V; Department of Biochemistry, Himalayan Institute of Medical Sciences, Dehradun 248140, India., Rao DN; Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. Electronic address: dnrao311@rediffmail.com.
Jazyk: angličtina
Zdroj: Biologicals : journal of the International Association of Biological Standardization [Biologicals] 2017 Sep; Vol. 49, pp. 81-85. Date of Electronic Publication: 2017 Aug 18.
DOI: 10.1016/j.biologicals.2017.08.005
Abstrakt: Major difficulty in development of dengue diagnostics is availability of suitable antigens. To overcome this, we made an attempt to develop a peptide based diagnosis which offers significant advantage over other methods. With the help of in silico methods, two epitopes were selected from envelope protein and three from NS1 protein of dengue virus. These were synthesized in combination as three multiple antigenic peptides (MAPs). We have tested 157 dengue positive sera confirmed for NS1 antigen. MAP1 showed 96.81% sera positive for IgM and 68.15% positive for IgG. MAP2 detected 94.90% IgM and 59.23% IgG positive sera. MAP3 also detected 96.17% IgM and 59.87% IgG positive sera. To the best of our knowledge this is the first study describing the use of synthetic multiple antigenic peptides for the diagnosis of dengue infection. This study describes MAPs as a promising tool for the use in serodiagnosis of dengue.
(Copyright © 2017 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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