Genetic variation of 15 autosomal STRs in a population sample of Bedouins residing in the area of the Fourth Nile Cataract, Sudan.

Autor: Ossowski A; Department of Forensic Genetics, Pomeranian Medical University, al. Powstańców Wlkp. 72, 70-111 Szczecin, Poland andoss@wp.pl , jpiatek@pum.edu.pl , pam_mir@wp.pl., Piatek J; Department of Forensic Genetics, Pomeranian Medical University, al. Powstańców Wlkp. 72, 70-111 Szczecin, Poland andoss@wp.pl , jpiatek@pum.edu.pl , pam_mir@wp.pl., Parafiniuk M; Department of Forensic Genetics, Pomeranian Medical University, al. Powstańców Wlkp. 72, 70-111 Szczecin, Poland andoss@wp.pl , jpiatek@pum.edu.pl , pam_mir@wp.pl., Pudlo A; Archaeological Museum in Gdansk, Mariacka 25/26, 80-958 Gdańsk Poland a.pudlo@archeologia.pl Department of Forensic Medicine, Medical University of Bialystok, Jana Kilinskiego 1, 15-089 Bialystok, Poland pepinski@umb.edu.pl , mskaw@o2.pl , michalszeremeta@gmail.com , anna.janica@umb.edu.pl., Pepinski W; Department of Forensic Medicine, Medical University of Bialystok, Jana Kilinskiego 1, 15-089 Bialystok, Poland pepinski@umb.edu.pl , mskaw@o2.pl , michalszeremeta@gmail.com , anna.janica@umb.edu.pl., Skawronska M; Department of Forensic Medicine, Medical University of Bialystok, Jana Kilinskiego 1, 15-089 Bialystok, Poland pepinski@umb.edu.pl , mskaw@o2.pl , michalszeremeta@gmail.com , anna.janica@umb.edu.pl., Szeremeta M; Department of Forensic Medicine, Medical University of Bialystok, Jana Kilinskiego 1, 15-089 Bialystok, Poland pepinski@umb.edu.pl , mskaw@o2.pl , michalszeremeta@gmail.com , anna.janica@umb.edu.pl., Niemcunowicz-Janica A; Department of Forensic Medicine, Medical University of Bialystok, Jana Kilinskiego 1, 15-089 Bialystok, Poland pepinski@umb.edu.pl , mskaw@o2.pl , michalszeremeta@gmail.com , anna.janica@umb.edu.pl., Soltyszewski I; Department of Criminalistics and Forensic Medicine, University of Warmia and Mazury, Warszawska 98, 10-702 Olsztyn, Poland.
Jazyk: angličtina
Zdroj: Anthropologischer Anzeiger; Bericht uber die biologisch-anthropologische Literatur [Anthropol Anz] 2017 Dec 01; Vol. 74 (4), pp. 263-268. Date of Electronic Publication: 2017 Aug 17.
DOI: 10.1127/anthranz/2017/0718
Abstrakt: Abstract: The purpose of the paper was to report allelic frequencies of 15 autosomal STR markers (AmpFlSTR NGM PCR Amplification Kit) for Bedouin inhabitants in the area of the Fourth Nile Cataract in Sudan, and compute commonly used population and forensic biostatistical parameters. Buccal swabs were collected from 117 unrelated individuals. DNA was extracted using DNA QIAamp ® DNA Mini Kit, and quantitated with Quantifiler Human Quantification Kit in a 7500 Real-Time PCR System. Amplification of 15 AmpFlSTR NGM PCR Kit loci was performed in PCR System 9700. Electrophoresis and typing were performed in 3130 Genetic Analyzer. Arlequin v3.5 software and PowerStats v1.2 spreadsheet were used for statistical calculations. The STR frequency distributions showed no deviations from HWE. The combined values of Matching Probability and Power of Exclusion are 1.77 × 10 -18 and 0.9999996, respectively. The average observed heterozygosity over 15 loci is 0.8069. Five different allelic microvariants were found. A significant linkage disequilibrium was observed in five pairs of loci . A 15 STR population database has been established for Sudanese Bedouins. The systems studied have been shown to be useful tool for personal identification in this population.
Databáze: MEDLINE
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