A CRISPR Activation Screen Identifies a Pan-avian Influenza Virus Inhibitory Host Factor.

Autor:	Heaton BE; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Kennedy EM; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Dumm RE; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Harding AT; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Sacco MT; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Sachs D; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA., Heaton NS; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA. Electronic address: nicholas.heaton@duke.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2017 Aug 15; Vol. 20 (7), pp. 1503-1512.
DOI:	10.1016/j.celrep.2017.07.060
Abstrakt:	Influenza A virus (IAV) is a pathogen that poses significant risks to human health. It is therefore critical to develop strategies to prevent influenza disease. Many loss-of-function screens have been performed to identify the host proteins required for viral infection. However, there has been no systematic screen to identify the host factors that, when overexpressed, are sufficient to prevent infection. In this study, we used CRISPR/dCas9 activation technology to perform a genome-wide overexpression screen to identify IAV restriction factors. The major hit from our screen, B4GALNT2, showed inhibitory activity against influenza viruses with an α2,3-linked sialic acid receptor preference. B4GALNT2 overexpression prevented the infection of every avian influenza virus strain tested, including the H5, H9, and H7 subtypes, which have previously caused disease in humans. Thus, we have used CRISPR/dCas9 activation technology to identify a factor that can abolish infection by avian influenza viruses. (Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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