Detectable HIV-RNA in semen of HIV controllers.

Autor: Chaix ML; APHP, Laboratoire de virologie, Hôpital Saint Louis, Paris, France.; INSERM U941, Université Paris Diderot, Paris, France., Boufassa F; INSERM CESP U1018, Faculté de Médecine Paris Sud, Le Kremlin-Bicêtre, France., Meyzer C; Centre d'Investigation Clinique Mère-Enfant, Hôpital Necker-Enfants Malades, Paris, France., Leruez-Ville M; APHP, Laboratoire de Virologie, Hôpital Necker-Enfants Malades, Paris, France., Mahjoub N; APHP, Laboratoire de virologie, Hôpital Saint Louis, Paris, France., Nere ML; APHP, Laboratoire de virologie, Hôpital Saint Louis, Paris, France., Genet P; Centre hospitalier Victor Dupouy, Service d'hématologie, Argenteuil, France., Duvivier C; Institut Pasteur, et Centre Médical Necker-Pasteur, Paris, France., Lascoux-Combes C; APHP, Hôpital Saint Louis, service de maladies infectieuses, Paris, France., Lambotte O; APHP, Service de Médecine Interne et d'Immunologie Clinique, Hôpital Bicêtre, Le Kremlin Bicêtre, France.; INSERM, U1184, Immunology of Viral Infections and Autoimmune Diseases, Le Kremlin-Bicêtre, France.; Université Paris Sud, UMR 1184, Le Kremlin-Bicêtre, France.; CEA, DSV/iMETI, IDMIT, Fontenay-aux-Roses, France., Ghosn J; Université Paris Descartes, Paris, France.; INSERM UMR-S 1136, Institut Pierre Louis d'Epidémiologie et de Santé Publique, Université Pierre et Marie Curie, Paris, France.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 Aug 16; Vol. 12 (8), pp. e0183376. Date of Electronic Publication: 2017 Aug 16 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0183376
Abstrakt: Background: Whether spontaneous low levels of HIV-1 RNA in blood plasma correlate with low levels of HIV-1 RNA in seminal plasma has never been investigated in HIV controller (HIC) men so far.
Methods: HIC men enrolled in the ANRS CODEX cohort were eligible for the present study if they had no symptoms of sexually transmitted infections (STI). Two paired samples of blood and semen were collected four weeks apart. HIV-RNA was quantified in blood plasma (bpVL) and in seminal plasma (spVL), and cell-associated HIV-DNA was quantified in peripheral blood mononuclear cells (PBMC) and in non-sperm cells (NSC). Spearman rho tests were used to estimate correlations between bpVL and spVL.
Results: Ten men were enrolled. At Day 0 (D0), spVL was detectable in four patients: 458; 552; 256 copies/mL and PCR signal detectable below limit of quantification (LoQ, 40 copies/mL). At Day 28 (D28), spVL was detectable in the same four participants in whom spVL was detectable at D0 with 582; 802; 752 and 50 copies/mL, respectively. HIV-DNA was detectable below LoQ in NSC of one patient at D0 visit. No patient had detectable HIV-DNA in NSC at D28 visit. At D0, bpVL and spVL were highly positively correlated (Spearman rho: 0.94; p = 0.0001). Similar results were found at D28.
Conclusion: We show that HIV-RNA can be detected in the semen of HIC men, with levels positively correlated with those measured concomitantly in blood plasma. HIC men should be aware of the risk of HIV genital shedding, especially if viral blips are reported.
Databáze: MEDLINE
Externí odkaz: