Bacteroidales recruit IL-6-producing intraepithelial lymphocytes in the colon to promote barrier integrity.

Autor: Kuhn KA; Department of Medicine, Division of Rheumatology, University of Colorado School of Medicine, Aurora, Colorado, USA.; Mucosal Inflammation Program, University of Colorado School of Medicine, Aurora, Colorado, USA., Schulz HM; Department of Medicine, Division of Rheumatology, University of Colorado School of Medicine, Aurora, Colorado, USA.; Mucosal Inflammation Program, University of Colorado School of Medicine, Aurora, Colorado, USA., Regner EH; Department of Medicine, Division of Gastroenterology, University of Colorado School of Medicine, Aurora, Colorado, USA., Severs EL; Department of Medicine, Division of Rheumatology, University of Colorado School of Medicine, Aurora, Colorado, USA.; Mucosal Inflammation Program, University of Colorado School of Medicine, Aurora, Colorado, USA., Hendrickson JD; Department of Medicine, Division of Rheumatology, University of Colorado School of Medicine, Aurora, Colorado, USA.; Mucosal Inflammation Program, University of Colorado School of Medicine, Aurora, Colorado, USA., Mehta G; Department of Medicine, Division of Rheumatology, University of Colorado School of Medicine, Aurora, Colorado, USA.; Mucosal Inflammation Program, University of Colorado School of Medicine, Aurora, Colorado, USA., Whitney AK; Mucosal Inflammation Program, University of Colorado School of Medicine, Aurora, Colorado, USA.; Department of Medicine, Division of Gastroenterology, University of Colorado School of Medicine, Aurora, Colorado, USA., Ir D; Department of Medicine, Division of Infectious Diseases, University of Colorado School of Medicine, Aurora, Colorado, USA., Ohri N; Department of Medicine, Division of Rheumatology, University of Colorado School of Medicine, Aurora, Colorado, USA.; Mucosal Inflammation Program, University of Colorado School of Medicine, Aurora, Colorado, USA., Robertson CE; Department of Medicine, Division of Infectious Diseases, University of Colorado School of Medicine, Aurora, Colorado, USA., Frank DN; Department of Medicine, Division of Infectious Diseases, University of Colorado School of Medicine, Aurora, Colorado, USA., Campbell EL; Mucosal Inflammation Program, University of Colorado School of Medicine, Aurora, Colorado, USA.; Department of Medicine, Division of Gastroenterology, University of Colorado School of Medicine, Aurora, Colorado, USA., Colgan SP; Mucosal Inflammation Program, University of Colorado School of Medicine, Aurora, Colorado, USA.; Department of Medicine, Division of Gastroenterology, University of Colorado School of Medicine, Aurora, Colorado, USA.
Jazyk: angličtina
Zdroj: Mucosal immunology [Mucosal Immunol] 2018 Mar; Vol. 11 (2), pp. 357-368. Date of Electronic Publication: 2017 Aug 16.
DOI: 10.1038/mi.2017.55
Abstrakt: Interactions between the microbiota and distal gut are important for the maintenance of a healthy intestinal barrier; dysbiosis of intestinal microbial communities has emerged as a likely contributor to diseases that arise at the level of the mucosa. Intraepithelial lymphocytes (IELs) are positioned within the epithelial barrier, and in the small intestine they function to maintain epithelial homeostasis. We hypothesized that colon IELs promote epithelial barrier function through the expression of cytokines in response to interactions with commensal bacteria. Profiling of bacterial 16S ribosomal RNA revealed that candidate bacteria in the order Bacteroidales are sufficient to promote IEL presence in the colon that in turn produce interleukin-6 (IL-6) in a MyD88 (myeloid differentiation primary response 88)-dependent manner. IEL-derived IL-6 is functionally important in the maintenance of the epithelial barrier as IL-6 -/- mice were noted to have increased paracellular permeability, decreased claudin-1 expression, and a thinner mucus gel layer, all of which were reversed by transfer of IL-6 +/+ IELs, leading to protection of mice in response to Citrobacter rodentium infection. Therefore, we conclude that microbiota provide a homeostatic role for epithelial barrier function through regulation of IEL-derived IL-6.
Databáze: MEDLINE
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