Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation.

Autor: Lee WT; Institute of Basic Medical Sciences, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan., Wu YN; Institute of Oral Medicine, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan., Chen YH; Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan., Wu SR; Institute of Oral Medicine, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan., Shih TM; Institute of Oral Medicine, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan., Li TJ; Institute of Basic Medical Sciences, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan., Yang LX; Institute of Basic Medical Sciences, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan., Yeh CS; Department of Chemistry, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan., Tsai PJ; Institute of Basic Medical Sciences, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan. peijtsai@mail.ncku.edu.tw.; Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan. peijtsai@mail.ncku.edu.tw.; Center of Infectious Disease and Signaling Research, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan. peijtsai@mail.ncku.edu.tw., Shieh DB; Institute of Basic Medical Sciences, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan. dbshieh@mail.ncku.edu.tw.; Institute of Oral Medicine, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan. dbshieh@mail.ncku.edu.tw.; Department of Stomatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 138 Sheng-Li Road, Tainan, 704, Taiwan. dbshieh@mail.ncku.edu.tw.; Advanced Optoelectronic Technology Center and Center for Micro/Nano Science and Technology, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan. dbshieh@mail.ncku.edu.tw.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Aug 15; Vol. 7 (1), pp. 8124. Date of Electronic Publication: 2017 Aug 15.
DOI: 10.1038/s41598-017-08387-y
Abstrakt: Clinical management of Clostridium difficile infection is still far from satisfactory as bacterial spores are resistant to many chemical agents and physical treatments. Certain types of nanoparticles have been demonstrated to exhibit anti-microbial efficacy even in multi-drug resistance bacteria. However, most of these studies failed to show biocompatibility to the mammalian host cells and no study has revealed in vivo efficacy in C. difficile infection animal models. The spores treated with 500 µg/mL Fe 3-δ O 4 nanoparticles for 20 minutes, 64% of the spores were inhibited from transforming into vegetative cells, which was close to the results of the sodium hypochlorite-treated positive control. By cryo-electron micro-tomography, we demonstrated that Fe 3-δ O 4 nanoparticles bind on spore surfaces and reduce the dipicolinic acid (DPA) released by the spores. In a C. difficile infection animal model, the inflammatory level triple decreased in mice with colonic C. difficile spores treated with Fe 3-δ O 4 nanoparticles. Histopathological analysis showed a decreased intense neutrophil accumulation in the colon tissue of the Fe 3-δ O 4 nanoparticle-treated mice. Fe 3-δ O 4 nanoparticles, which had no influence on gut microbiota and apparent side effects in vivo, were efficacious inhibitors of C. difficile spore germination by attacking its surface and might become clinically feasible for prophylaxis and therapy.
Databáze: MEDLINE
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