An Early Chick Embryo Culture Device for Extended Continuous Observation.

Autor:	Sydow HG; Anatomy and Embryology, University Medical Centre, Kreuzbergring 36, 37075, Göttingen, Germany., Pieper T; Anatomy and Embryology, University Medical Centre, Kreuzbergring 36, 37075, Göttingen, Germany., Viebahn C; Anatomy and Embryology, University Medical Centre, Kreuzbergring 36, 37075, Göttingen, Germany., Tsikolia N; Anatomy and Embryology, University Medical Centre, Kreuzbergring 36, 37075, Göttingen, Germany. nikoloz.tsikolia@med.uni-goettingen.de.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1650, pp. 309-317.
DOI:	10.1007/978-1-4939-7216-6_21
Abstrakt:	Appropriate mechanical tension of the vitelline membrane as the culture substrate for the early chick embryo is frequently reported to be required for successful in vitro development. Here we describe a modified device, made of anodized aluminum, for in vitro culture which we used for studies of left-right symmetry breaking with emphasis on morphology and gene expression as readouts. The technique allows for easy, high-throughput tissue handling and provides a suitable tension in a stable and easily reproducible manner proven to be suitable for correct molecular left-right patterning and heart looping after long-term culture.
Databáze:	MEDLINE
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