Synergy between 5-HT 4 receptor stimulation and phosphodiesterase 4 inhibition in facilitating acetylcholine release in human large intestinal circular muscle.

Autor: Pauwelyn V; Department of Pharmacology - Heymans Institute, Ghent University, Ghent, Belgium., Ceelen W; Department of Gastrointestinal Surgery, Ghent University Hospital, Ghent, Belgium., Lefebvre RA; Department of Pharmacology - Heymans Institute, Ghent University, Ghent, Belgium.
Jazyk: angličtina
Zdroj: Neurogastroenterology and motility [Neurogastroenterol Motil] 2018 Feb; Vol. 30 (2). Date of Electronic Publication: 2017 Aug 10.
DOI: 10.1111/nmo.13162
Abstrakt: Background: Gastroprokinetic properties of 5-HT 4 receptor agonists, such as prucalopride, are attributed to activation of 5-HT 4 receptors on cholinergic nerves innervating smooth muscle in the gastrointestinal smooth muscle layer, increasing acetylcholine release and muscle contraction. In porcine stomach and colon, phosphodiesterase (PDE) 4 has been shown to control the signaling pathway of these 5-HT 4 receptors. The aim of this study was to investigate the PDE-mediated control of these 5-HT 4 receptors in human large intestine.
Methods: Circular smooth muscle strips were prepared from human large intestine; after incubation with [³H]-choline, electrically induced tritium outflow was determined as a measure for acetylcholine release. The influence of PDE inhibition on the facilitating effect of prucalopride on electrically induced acetylcholine release was studied.
Key Results: The non-selective PDE inhibitor IBMX enhanced the facilitating effect of prucalopride on electrically induced acetylcholine release. The selective inhibitors vinpocetine (PDE1), EHNA (PDE2) and cilostamide (PDE3) did not influence, while rolipram and roflumilast (PDE4) enhanced the prucalopride-induced facilitation to the same extent as IBMX.
Conclusions & Inferences: In human large intestinal circular muscle, the intracellular pathway of 5-HT 4 receptors facilitating cholinergic neurotransmission to large intestinal circular smooth muscle is controlled by PDE4. If the synergy between 5-HT 4 receptor agonism and PDE4 inhibition is confirmed in a functional assay with electrically induced cholinergic contractions of human large intestinal circular smooth muscle strips, combination of a selective 5-HT 4 receptor agonist with a selective PDE4 inhibitor might enhance the in vivo prokinetic effect of the 5-HT 4 receptor agonist in the large intestine.
(© 2017 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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